A set of vertices S is a determining set of a graph G if every automorphism of G is uniquely determined by its action on S. The determining number of G is the minimum cardinality of a determining set of G. This paper studies the determining number of Kneser graphs. First, we compute the determining number of a wide range of Kneser graphs, concretely K-n:k with n >= k(k + 1)/2 + 1. In the language of group theory, these computations provide exact values for the base size of the symmetric group S-n acting on the k-subsets of {1,...,n}. Then, we establish for which Kneser graphs K n : k the determining number is equal to n - k, answering a question posed by Boutin. Finally, we find all Kneser graphs with fixed determining number 5, extending the study developed by Boutin for determining number 2, 3 or 4.