Increased expression of adrenomedullin 2/intermedin in rat hearts with congestive heart failure

被引：39

作者：

Hirose, Takuo ^{[1
]}

Totsune, Kazuhito ^{[1
,2
]}

Mori, Nobuyoshi ^{[3
]}

Morimoto, Ryo ^{[4
]}

Hashimoto, Masahiro ^{[1
]}

Nakashige, Yukiko ^{[1
]}

Metoki, Hirohito ^{[1
,5
]}

Asayama, Kei ^{[2
]}

Kikuya, Masahiro ^{[1
]}

Ohkubo, Takayoshi ^{[2
,6
]}

Hashimoto, Junichiro ^{[2
,6
]}

Sasano, Hironobu ^{[7
]}

Kohzuki, Masahiro ^{[3
]}

Takahashi, Kazzuhiro ^{[2
,8
]}

Imai, Yutaka ^{[1
,2
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, 21st Ctr Excellence Program, Comprehens Res & Educ Ctr Planning Drug Dev & Cli, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] Tohoku Univ, Sch Med, Dept Internal Med & Rehabil Sci, Aoba Ku, Sendai, Miyagi 9808574, Japan

[4] Tohoku Univ, Sch Med, Dept Med, Aoba Ku, Sendai, Miyagi 9808574, Japan

[5] Tohoku Univ, Sch Med, Dept Med Genet, Aoba Ku, Sendai, Miyagi 9808574, Japan

[6] Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Planning Drug Dev & Clin Evaluat, Aoba Ku, Sendai, Miyagi 9808575, Japan

[7] Tohoku Univ, Sch Med, Dept Pathol, Aoba Ku, Sendai, Miyagi 9808574, Japan

[8] Tohoku Univ, Grad Sch Med, Dept Endocrinol & Appl Med Sci, Aoba Ku, Sendai, Miyagi 9808575, Japan

来源：

EUROPEAN JOURNAL OF HEART FAILURE | 2008年 / 10卷 / 09期

关键词：

Adrenomedullin; 2/Intermedin; Heart failure; RT-PCR; Immunohistochemistry;

D O I：

10.1016/j.ejheart.2008.06.020

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide family. To investigate the pathophysiological role of AM2/IMD in heart failure, we examined the expression of AM2/IMD, adrenomedullin (AM) and receptor complex components (calcitonin receptor-like receptor, three types of receptor activity-modifying proteins) by quantitative RT-PCR and immunohistochemistry in the hearts and kidneys of rats with congestive heart failure (CHF). Significantly increased levels of AM2/IMD mRNA were found in the atrium, right ventricle, non-infarcted part of the left ventricle and the infarcted part of the left ventricle of CHF rats, compared with sham operated rats (about 2.8-fold, 1.7-fold, 1.7-fold and 2.5-fold, respectively). Expression levels of mRNA encoding AM and the receptor complex components were also increased in the hearts of CHF rats. In a separate experiment, AM2/IMD mRNA levels in the heart did not differ between Wistar-Kyoto and spontaneously hypertensive rats. In both sham operated and CHF rats, the myocardium was diffusely immunostained with AM2/IMD. The fibrotic infarcted layer was not immunostained with AM2/IMD but was surrounded by positively immunostained myocardial layers. These findings suggest that the expression of AM2/IMD is enhanced in the failing heart, and AM2/IMD has a certain pathophysiological role in heart failure. (C) 2008 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.

引用

页码：840 / 849

页数：10

共 30 条

[1] Intermedin, a novel calcitonin family peptide that exists in teleosts as well as in mammals: A comparison with other calcitonin/intermedin family peptides in vertebrates [J].