A novel high-sensitivity electrochemiluminescence (ECL) sandwich immunoassay for the specific quantitative measurement of plasma glucagon

被引：22

作者：

Sloan, John H. ^{[1
]}

Siegel, Robert W. ^{[1
]}

Ivanova-Cox, Yvelina T. ^{[1
]}

Watson, David E. ^{[1
]}

Deeg, Mark A. ^{[1
]}

Konrad, Robert J. ^{[1
]}

机构：

[1] Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA

来源：

CLINICAL BIOCHEMISTRY | 2012年 / 45卷 / 18期

关键词：

Glucagon; Glucose; Diabetes; Insulin; Peptide; Immunoassay; ALPHA-CELL-FUNCTION; RECEPTOR ANTAGONISTS; POSTPRANDIAL HYPERGLYCEMIA; POTENT; RADIOIMMUNOASSAY; OPTIMIZATION; ANTIBODIES; DISPLAY;

D O I：

10.1016/j.clinbiochem.2012.07.111

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: To develop a novel, dual-monoclonal sandwich immunoassay with superior sensitivity that provides a rapid and convenient method for measuring glucagon. Glucagon is a 29-amino acid polypeptide hormone produced in the pancreas by the a-cells of the islets of Langerhans. Working in concert with insulin, glucagon is involved in regulating circulating glucose concentrations. Design and methods: The immunoassay utilizes Meso Scale Discovery (MSD) electrochemiluminescence (ECL) technology and two affinity-optimized monoclonal antibodies. A series of experiments was performed to determine the linear range of the assay and to evaluate sensitivity, accuracy, recovery, precision, and linearity. Results: The sandwich assay was specific for glucagon and did not recognize the closely related peptide oxyntomodulin or other incretin peptides. The assay demonstrated excellent recovery, precision, and linearity, and a broad dynamic range of 0.14 pmol/L to 1950 pmol/L In addition, assay results were highly correlated with those obtained using a previously described competitive RIA employing polyclonal antiserum. Conclusion: The use of affinity-optimized monoclonal antibodies in a sandwich immunoassay format provides a robust, sensitive, and convenient method for measuring concentrations of glucagon that is highly sensitive and specific. This immunoassay should help to improve our understanding of the role of glucagon in the regulation of glucose metabolism. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：1640 / 1644

页数：5

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