Expression of an antifungal peptide in Saccharomyces:: A new approach for biological control of the postharvest disease caused by Colletotrichum coccodes

被引：38

作者：

Jones, RW

Prusky, D ^{[1
]}

机构：

[1] USDA ARS, Vegetable Lab, Henry A Wallace Beltsville Agr Ctr, Beltsville, MD 20705 USA

[2] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Prod, Inst Technol & Storage Agr Prod, IL-50250 Bet Dagan, Israel

来源：

PHYTOPATHOLOGY | 2002年 / 92卷 / 01期

关键词：

antifungal peptides; biocontrol mechanism;

D O I：

10.1094/PHYTO.2002.92.1.33

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cecropin A-based peptide inhibited germination of Colletotrichum coccodes at 50 muM. The DNA sequence encoding the peptide was cloned in pRS413, using the Saccharomyces cerevisiae invertase leader sequence for secretion of the peptide, and expressed in yeast. Yeast transformants inhibited the growth of germinated C, coccodes spores and inhibited decay development caused by C. coccodes in tomato fruits. Expression of the antifungal peptide in yeast therefore represents a new approach for the biological control of postharvest diseases.

引用

页码：33 / 37

页数：5

共 28 条

[1] [Anonymous], BIOL CONTROL POSTHAR
[2] BENARIE R, 1991, BIOL CONTROL POSTHAR, P100
[3] PEPTIDE ANTIBIOTICS AND THEIR ROLE IN INNATE IMMUNITY
BOMAN, HG
[J]. ANNUAL REVIEW OF IMMUNOLOGY, 1995, 13 : 61 - 92
[4] BOMAN HG, 1981, TRENDS BIOCHEM SCI, V6, P306, DOI 10.1146/annurev.mi.41.100187.000535
[5] THE SECRETED FORM OF INVERTASE IN SACCHAROMYCES-CEREVISIAE IS SYNTHESIZED FROM MESSENGER-RNA ENCODING A SIGNAL SEQUENCE
CARLSON, M
TAUSSIG, R
KUSTU, S
BOTSTEIN, D
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (03) : 439 - 447
[6] Cecropin A-derived peptides are potent inhibitors of fungal plant pathogens
Cavallarin, L
Andreu, D
Segundo, BS
[J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (03) : 218 - 227
[7] POSTHARVEST BIOCONTROL OF GREEN AND BLUE MOLD AND SOUR ROT OF CITRUS-FRUIT BY DEBARYOMYCES-HANSENII
CHALUTZ, E
WILSON, CL
[J]. PLANT DISEASE, 1990, 74 (02) : 134 - 137
[8] Control of postharvest pear diseases using natural saprophytic yeast colonists and their combination with a low dosage of thiabendazole
ChandGoyal, T
Spotts, RA
[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY, 1996, 7 (1-2) : 51 - 64
[9] Commercial testing of aspire: A yeast preparation for the biological control of postharvest decay of citrus
Droby, S
Cohen, L
Daus, A
Weiss, B
Horev, B
Chalutz, E
Katz, H
Keren-Tzur, M
Shachnai, A
[J]. BIOLOGICAL CONTROL, 1998, 12 (02) : 97 - 101
[10] Droby S., 1991, Postharvest News and Information, V2, P169

← 1 2 3 →