Expression of an antifungal peptide in Saccharomyces:: A new approach for biological control of the postharvest disease caused by Colletotrichum coccodes

被引：39

作者：

Jones, RW

Prusky, D ^{[1
]}

机构：

[1] USDA ARS, Vegetable Lab, Henry A Wallace Beltsville Agr Ctr, Beltsville, MD 20705 USA

[2] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Prod, Inst Technol & Storage Agr Prod, IL-50250 Bet Dagan, Israel

来源：

PHYTOPATHOLOGY | 2002年 / 92卷 / 01期

关键词：

antifungal peptides; biocontrol mechanism;

D O I：

10.1094/PHYTO.2002.92.1.33

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cecropin A-based peptide inhibited germination of Colletotrichum coccodes at 50 muM. The DNA sequence encoding the peptide was cloned in pRS413, using the Saccharomyces cerevisiae invertase leader sequence for secretion of the peptide, and expressed in yeast. Yeast transformants inhibited the growth of germinated C, coccodes spores and inhibited decay development caused by C. coccodes in tomato fruits. Expression of the antifungal peptide in yeast therefore represents a new approach for the biological control of postharvest diseases.

引用

页码：33 / 37

页数：5

共 28 条

[1]

[Anonymous], BIOL CONTROL POSTHAR

[2]

BENARIE R, 1991, BIOL CONTROL POSTHAR, P100

[3] PEPTIDE ANTIBIOTICS AND THEIR ROLE IN INNATE IMMUNITY [J].