Detection by PCR of adhesins genes and slime production in clinical Staphylococcus aureus

被引：46
作者：
Zmantar, Tarek [1 ]
Chaieb, Kamel [1 ]
Makni, Hela [1 ]
Miladi, Hannene [1 ]
Ben Abdallah, Fethi [1 ]
Mahdouani, Kacem
Bakhrouf, Amina [1 ]
机构：
[1] Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir, Tunisia
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2008年 / 48卷 / 04期
关键词：
adhesins genes (fnbA; cna and clfA); auricular infection; ica loci; slime production; Staphylococcus aureus;
D O I：
10.1002/jobm.200700289
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to detect the adhesins genes (icaA, icaD, fnbA, cna, ClfA) Polymerase Chain Reaction was used. Qualitative biofilm production of S. aureus using CRA plates revealed that 56.5% of strains were slime producers. In addition 78.26% of strains were icaA and icaD positive. While the fnbA gene was present in 76.1% of isolated strains. Furthermore, 56.5% of strains have the cna gene and 30.4% were clfA positives. Overall this study confirms the presence of fnbA and icaA/icaD genes in the majority of studies S. aureus strains isolated from Staphylococcal sepsis.
引用
收藏
页码：308 / 314
页数：7
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