Total flavonoids from Rosa Laevigata Michx fruit attenuates hydrogen peroxide induced injury in human umbilical vein endothelial cells

被引:53
作者
Jia, Yannan [1 ]
Ji, Liu [1 ]
Zhang, Shuai [1 ]
Xu, Lina [1 ]
Yin, Lianhong [1 ]
Li, Lei [1 ]
Zhao, Yanyan [1 ]
Peng, Jinyong [1 ]
机构
[1] Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
关键词
Total flavonoids; Rosa laevigata Michx; Human umbilical vein endothelial cells; H2O2-induced damage; NITRIC-OXIDE; APOPTOSIS; IDENTIFICATION; MITOCHONDRIA; DEATH;
D O I
10.1016/j.fct.2012.06.047
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The aim of the present study was to evaluate the protective effect and possible mechanism of the total flavonoids (TFs) from Rosa Laevigata Michx fruit (RLMF) against hydrogen peroxide (H2O2) induced damage in human umbilical vein endothelial cells (HUVECs). The cell injury caused by H2O2 was protected by pretreatment with the TFs for 1 h. Compared with the model group, the TFs decreased S phase cells, suppressed nuclear morphological damage, inhibited the collapse of mitochondrial membrane potentials (Delta Psi m), attenuated excessive reactive oxygen species generation, reduced glutathione depletion, impacted the mitochondrial morphology change, decreased caspase-3, -9 activities, and decreased fragmented DNA. Further mechanism investigation showed that the TFs could increase the protein expressions of Procaspase-3, Bcl-2, and decrease the expressions of Bak, Bax, Bid and p53. Generally, the TFs from RLMF is an effective natural product for the treatment of cardiovascular and cerebrovascular diseases. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3133 / 3141
页数:9
相关论文
共 37 条
[1]  
[Anonymous], CARD DIS
[2]  
China Pharmacopoeia Committee, 2005, PHARM PEOPL REP CHIN, P153
[3]  
Díez JMB, 2005, REV ESP CARDIOL, V58, P367, DOI 10.1157/13073893
[4]   Nitric oxide - an endothelial cell survival factor [J].
Dimmeler, S ;
Zeiher, AM .
CELL DEATH AND DIFFERENTIATION, 1999, 6 (10) :964-968
[5]   Triterpenes from fruits of Rosa laevigata [J].
Gao, Pin-Yi ;
Li, Ling-Zhi ;
Peng, Ying ;
Piao, Shu-Juan ;
Zeng, Na ;
Lin, Hou-Wen ;
Song, Shao-Jiang .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2010, 38 (03) :457-459
[6]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[7]   Synthetic retinoid CD437 induces mitochondria-mediated apoptosis in hepatocellular carcinoma cells [J].
Gonda, Kazue ;
Tsuchiya, Hiroyuki ;
Sakabe, Tomohiko ;
Akechi, Yuji ;
Ikeda, Remina ;
Nishio, Ren ;
Terabayashi, Kei ;
Ishii, Kyoko ;
Matsumi, Yoshiaki ;
Ashla, An Afida ;
Okamoto, Hideharu ;
Takubo, Kazuko ;
Matsuoka, Saori ;
Watanabe, Yumi ;
Hoshikawa, Yoshiko ;
Kurimasa, Akihiro ;
Shiota, Goshi .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 370 (04) :629-633
[8]  
Huang Y.X., 2009, RES PRACT CHIN MED, V23, P32
[9]   ROS-generating mitochondrial DNA mutations can regulate tumor cell metastasis [J].
Ishikawa, Kaori ;
Takenaga, Keizo ;
Akimoto, Miho ;
Koshikawa, Nobuko ;
Yamaguchi, Aya ;
Imanishi, Hirotake ;
Nakada, Kazuto ;
Honma, Yoshio ;
Hayashi, Jun-Ichi .
SCIENCE, 2008, 320 (5876) :661-664
[10]   CULTURE OF HUMAN ENDOTHELIAL CELLS DERIVED FROM UMBILICAL VEINS - IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGICAL CRITERIA [J].
JAFFE, EA ;
NACHMAN, RL ;
BECKER, CG ;
MINICK, CR .
JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (11) :2745-2756