RETRACTED: miR-135 promotes proliferation and stemness of oesophageal squamous cell carcinoma by targeting RERG (Retracted Article)

被引：24

作者：

Zhang, Yan ^{[1
]}

Ren, Shuang ^{[2
]}

Yuan, Fengfeng ^{[1
]}

Zhang, Kaishang ^{[1
]}

Fan, Yingying ^{[1
]}

Zheng, Shaozhong ^{[1
]}

Gao, Zhen ^{[1
]}

Zhao, Jia ^{[1
]}

Mu, Teng ^{[1
]}

Zhao, Song ^{[1
]}

Shang, AnQuan ^{[3
]}

Li, Xiangnan ^{[1
]}

Jie, Ying ^{[4
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Thorac Surg, 1st Jianshe East Rd, Zhengzhou 450052, Henan, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 2, Dept Oncol, Zhengzhou, Henan, Peoples R China

[3] Tongji Univ, Tongji Hosp, Sch Med, Dept Lab Med, 389 Xin Cun Rd, Shanghai 200065, Peoples R China

[4] Xuyi Peoples Hosp, Dept Clin Res Ctr, 28 Hongwu Rd, Xuyi 211700, Jiangsu, Peoples R China

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2018年 / 46卷

关键词：

Oesophageal squamous cell carcinoma; microRNA; GTPase signalling; RAS-like oestrogen-regulated growth inhibitor; BREAST-CANCER; MICRORNA EXPRESSION; RAS; CHEMORADIATION; EPIDEMIOLOGY; ACTIVATION; MECHANISMS; STRATEGIES; MANAGEMENT; PATHWAY;

D O I：

10.1080/21691401.2018.1483379

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNA (miRNA) plays an important role in tumourigenesis and cancer development by regulating oncogenes or tumour suppressor that are implicated in cell cycle, cell mobility and even cell senescence. Due to the resistance to enzymes that could degrade nucleotides, miRNAs have been considered proper for diagnosis and prognosis evaluation of cancer. The present study was designed to investigate miRNA associated with ESCC and identified effective miRNAs, which could serve as biomarker or targets. We first performed miRNA profiling to identify a subset of dysregulated miRNAs in ESCC. miR-135, miR-451 and miR-186 were the most differentially expressed miRNAs. Subsequent RT-PCR validated that miR-135 was upregulated in ESCC cell lines TE2 and TE9, implying the promise as a prognostic and diagnostic marker. The Cox regression analysis suggests the correlation of miR-135 expression and tumour stages. Survival analysis demonstrated metastatic samples largely have higher miR-135 expression. Downregulation of miR-135 suppressed proliferation and invasion of TE2 and TE9 cell lines. Subsequent target prediction combined with functional enrichment analysis identified "Small GTPase superfamily" that are possibly targeted by miR-135, which offers candidates for further investigation. Finally, RERG was identified as a target of miR-135. Downregulation of RERG could inhibit the cell proliferation and sphere formation ability of TE2 and TE9. Taken together, miR-135 was proved to promote tumour development of ESCC, which promises the prospect of using miR-135 as a biomarker indicator in diagnosis and prognosis.

引用

页码：1210 / 1219

页数：10

共 48 条

[1] Predicting effective microRNA target sites in mammalian mRNAs [J].

Agarwal, Vikram ;

Bell, George W. ;

Nam, Jin-Wu ;

Bartel, David P. .

ELIFE, 2015, 4

[2] MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[3] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[4] p53-mediated activation of miRNA34 candidate tumor-suppressor genes [J].

Bommer, Guido T. ;

Gerin, Isabelle ;

Feng, Ying ;

Kaczorowski, Andrew J. ;

Kuick, Rork ;

Love, Robert E. ;

Zhai, Yali ;

Giordano, Thomas J. ;

Qin, Zhaohui S. ;

Moore, Bethany B. ;

MacDougald, Ormond A. ;

Cho, Kathleen R. ;

Fearon, Eric R. .

CURRENT BIOLOGY, 2007, 17 (15) :1298-1307

[5] Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers [J].

Calin, GA ;

Sevignani, C ;

Dan Dumitru, C ;

Hyslop, T ;

Noch, E ;

Yendamuri, S ;

Shimizu, M ;

Rattan, S ;

Bullrich, F ;

Negrini, M ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (09) :2999-3004

[6] MicroRNA signatures in human cancers [J].

Calin, George A. ;

Croce, Carlo M. .

NATURE REVIEWS CANCER, 2006, 6 (11) :857-866

[7] miRNA-135a promotes breast cancer cell migration and invasion by targeting HOXA10 [J].

Chen, Yating ;

Zhang, Jin ;

Wang, Huijun ;

Zhao, Jiayi ;

Xu, Cheng ;

Du, Yingying ;

Luo, Xin ;

Zheng, Fengyun ;

Liu, Rui ;

Zhang, Hongwei ;

Ma, Duan .

BMC CANCER, 2012, 12

[8] DAVID: Database for annotation, visualization, and integrated discovery [J].

Dennis, G ;

Sherman, BT ;

Hosack, DA ;

Yang, J ;

Gao, W ;

Lane, HC ;

Lempicki, RA .

GENOME BIOLOGY, 2003, 4 (09)

[9]

Duan Xiao-Feng, 2014, Cancer Biology Medicine, V11, P191, DOI 10.7497/j.issn.2095-3941.2014.03.005

[10] Temporal Trends in Long-Term Survival and Cure Rates in Esophageal Cancer A SEER Database Analysis [J].

Dubecz, Attila ;

Gall, Isabell ;

Solymosi, Norbert ;

Schweigert, Michael ;

Peters, Jeffrey H. ;

Feith, Marcus ;

Stein, Hubert J. .

JOURNAL OF THORACIC ONCOLOGY, 2012, 7 (02) :443-447

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