Solvent Properties of Water in Aqueous Solutions of Elastin-Like Polypeptide

被引：21

作者：

Ferreira, Luisa A. ^{[1
]}

Cole, James T. ^{[2
]}

Reichardt, Christian ^{[3
]}

Holland, Nolan B. ^{[2
]}

Uversky, Vladimir N. ^{[4
,5
,6
,7
,8
]}

Zaslavsky, Boris Y. ^{[1
]}

机构：

[1] Analiza Inc, Cleveland, OH 44114 USA

[2] Cleveland State Univ, Dept Chem & Biomed Engn, Cleveland, OH 44115 USA

[3] Univ Marburg, Dept Chem, D-35032 Marburg, Germany

[4] Univ S Florida, Morsani Coll Med, Dept Mol Med, Tampa, FL 33612 USA

[5] Univ S Florida, Morsani Coll Med, USF Hlth Byrd Alzheimers Res Inst, Tampa, FL 33612 USA

[6] Russian Acad Sci, Inst Biol Instrumentat, Pushchino 142292, Moscow Region, Russia

[7] King Abdulaziz Univ, Fac Sci, Dept Biol, Jeddah 21589, Saudi Arabia

[8] Russian Acad Sci, Inst Cytol, Lab Struct Dynam Stabil & Folding Prot, St Petersburg 194064, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

SOLVATOCHROMIC COMPARISON METHOD; INVERSE TEMPERATURE TRANSITION; FREE-ENERGY TRANSDUCTION; PHASE-TRANSITION; HOFMEISTER ANIONS; PROTEINS; SCALE; PURIFICATION; CHEMISTRY; MECHANISM;

D O I：

10.3390/ijms160613528

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The phase-transition temperatures of an elastin-like polypeptide (ELP) with the (GVGVP)(40) sequence and solvent dipolarity/polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts (Na2SO4, NaCl, NaClO4, and NaSCN) and various osmolytes (sucrose, sorbitol, trehalose, and trimethylamine N-oxide (TMAO)). All osmolytes decreased the ELP phase-transition temperature, whereas NaCl and Na2SO4 decreased, and NaSCN and NaClO4 increased it. The determined phase-transition temperatures may be described as a linear combination of the solvent's dipolarity/polarizability and hydrogen-bond donor acidity. The linear relationship established for the phase-transition temperature in the presence of salts differs quantitatively from that in the presence of osmolytes, in agreement with different (direct and indirect) mechanisms of the influence of salts and osmolytes on the ELP phase-transition temperature.

引用

页码：13528 / 13547

页数：20

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