Can amino-functionalized carbon nanotubes carry functional nerve growth factor?

被引：21

作者：

Chen, Wen ^{[1
,2
]}

Xiong, Qing ^{[2
]}

Ren, Quanxia ^{[2
]}

Guo, Yake ^{[2
]}

Li, Gao ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Pharmacol, Tongji Med Coll, Wuhan 430030, Hubei Province, Peoples R China

[2] Shihezi Univ, Sch Pharm, Shihezi, Xinjiang Uygur, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2014年 / 9卷 / 03期

基金：

中国国家自然科学基金;

关键词：

nerve regeneration; spinal cord injury; carbon nanotubes; amino functionalization; nerve growth factor; cytotoxicity; biological activity; dispersibility; PC12; cells; chick embryo dorsal root ganglion; NSFC grant; neural regeneration; PROTEIN-KINASE-C; DELIVERY; TRANSPORTERS; DISSOLUTION; NEURONS; DNA; INTERNALIZATION; ACTIVATION; CELLS;

D O I：

10.4103/1673-5374.128225

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Carbon nanotubes can carry protein into cells to induce biological effects. Amino-functionalized carbon nanotubes are soluble and biocompatible, have high reactivity and low toxicity, and can help promote nerve cell growth. In this study, amino-functionalized ethylenediamine-treated multi-walled carbon nanotubes were used to prepare carbon nanotubes-nerve growth factor complexes by non-covalent grafting. The physicochemical properties, cytotoxicity to PC12 and chick embryo dorsal root ganglion, and biological activity of the carbon nanotubes-nerve growth factor complexes were investigated. The results showed that amino functionalization improved carbon nanotubes-nerve growth factor complex dispersibility, reduced their toxicity to PC12 cells, and promoted PC12 cell differentiation and chick embryo dorsal root ganglion.

引用

页码：285 / 292

页数：8

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