The search for new antigenic targets in myasthenia gravis

被引：63

作者：

Cossins, Judith ^{[1
]}

Belaya, Katsiaryna ^{[1
]}

Zoltowska, Katarzyna ^{[1
]}

Koneczny, Inga ^{[1
]}

Maxwell, Susan ^{[1
]}

Jacobson, Leslie ^{[2
]}

Leite, Maria Isabel ^{[2
]}

Waters, Patrick ^{[2
]}

Vincent, Angela ^{[2
]}

Beeson, David ^{[1
]}

机构：

[1] John Radcliffe Hosp, Weatherall Inst Mol Med, Nuffield Dept Clin Neurosci, Oxford OX3 9DS, England

[2] John Radcliffe Hosp, Nuffield Dept Clin Neurosci, Oxford OX3 9DS, England

来源：

MYASTHENIA GRAVIS AND RELATED DISORDERS II | 2012年 / 1275卷

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

myasthenia; autoantibodies; AChR; Lrp4; agrin; ColQ; MuSK; ACETYLCHOLINE-RECEPTOR; NEUROMUSCULAR-JUNCTION; COLQ MUTATION; KINASE MUSK; PROTEIN; ANTIBODIES; AUTOANTIBODIES; AGRIN; LRP4; GENE;

D O I：

10.1111/j.1749-6632.2012.06833.x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Around 80% of myasthenia gravis patients have antibodies against the acetylcholine receptor, and 0-60% of the remaining patients have antibodies against the muscle-specific tyrosine kinase, MuSK. Another recently identified antigen is low-density lipoprotein receptor-related protein 4 (Lrp4). To improve the existing assays and widen the search for new antigenic targets, we have employed cell-based assays in which candidate target proteins are expressed on the cell surface of transfected cells and probed with patient sera. These assays, combined with use of myotube cultures to explore the effects of the antibodies, enable us to begin to identify new antigenic targets and test antibody pathogenicity in vitro.

引用

页码：123 / 128

页数：6

共 38 条

[11] Auto-antibodies to the receptor tyrosine kinase MuSK in patients with myasthenia gravis without acetylcholine receptor antibodies [J].

Hoch, W ;

McConville, J ;

Helms, S ;

Newsom-Davis, J ;

Melms, A ;

Vincent, A .

NATURE MEDICINE, 2001, 7 (03) :365-368

[12] Heparin inhibits acetylcholine receptor aggregation at two distinct steps in the agrin-induced pathway [J].

Hopf, C ;

Hoch, W .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1997, 9 (06) :1170-1177

[13] Antibodies to Kv1 potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan's syndrome and acquired neuromyotonia [J].

Irani, Sarosh R. ;

Alexander, Sian ;

Waters, Patrick ;

Kleopa, Kleopas A. ;

Pettingill, Philippa ;

Zuliani, Luigi ;

Peles, Elior ;

Buckley, Camilla ;

Lang, Bethan ;

Vincent, Angela .

BRAIN, 2010, 133 :2734-2748

[14] Presence and Pathogenic Relevance of Antibodies to Clustered Acetylcholine Receptor in Ocular and Generalized Myasthenia Gravis [J].

Jacob, Saiju ;

Viegas, Stuart ;

Leite, Maria Isabel ;

Webster, Richard ;

Cossins, Judith ;

Kennett, Robin ;

Hilton-Jones, David ;

Morgan, B. Paul ;

Vincent, Angela .

ARCHIVES OF NEUROLOGY, 2012, 69 (08) :994-1001

[15] Anti-MuSK autoantibodies block binding of collagen Q to MuSK [J].

Kawakami, Y. ;

Ito, M. ;

Hirayama, M. ;

Sahashi, K. ;

Ohkawara, B. ;

Masuda, A. ;

Nishida, H. ;

Mabuchi, N. ;

Engel, A. G. ;

Ohno, K. .

NEUROLOGY, 2011, 77 (20) :1819-1826

[16] Lrp4 Is a Receptor for Agrin and Forms a Complex with MuSK [J].

Kim, Natalie ;

Stiegler, Amy L. ;

Cameron, Thomas O. ;

Hallock, Peter T. ;

Gomez, Andrea M. ;

Huang, Julie H. ;

Hubbard, Stevan R. ;

Dustin, Michael L. ;

Burden, Steven J. .

CELL, 2008, 135 (02) :334-342

[17] MuSK-positive myasthenia gravis is rare in the Polish population [J].

Kostera-Pruszczyk, A. ;

Kaminska, A. ;

Dutkiewicz, M. ;

Emeryk-Szajewska, B. ;

Strugalska-Cynowska, M. H. ;

Vincent, A. ;

Kwiecinski, H. .

EUROPEAN JOURNAL OF NEUROLOGY, 2008, 15 (07) :720-724

[18] Autoimmune disorders of the neuromuscular junction [J].

Lang, Bethan ;

Vincent, Angela .

CURRENT OPINION IN PHARMACOLOGY, 2009, 9 (03) :336-340

[19] IgG1 antibodies to acetylcholine receptors in 'seronegative' myasthenia gravis [J].

Leite, Maria Isabel ;

Jacob, Saiju ;

Viegas, Stuart ;

Cossins, Judy ;

Clover, Linda ;

Morgan, B. Paul ;

Beeson, David ;

Willcox, Nick ;

Vincent, Angela .

BRAIN, 2008, 131 :1940-1952

[20] LG2 agrin mutation causing severe congenital myasthenic syndrome mimics functional characteristics of non-neural (z-) agrin [J].

Maselli, Ricardo A. ;

Fernandez, Jose M. ;

Arredondo, Juan ;

Navarro, Carmen ;

Maian Ngo ;

Beeson, David ;

Cagney, Orla ;

Williams, D. Colette ;

Wollmann, Robert L. ;

Yarov-Yarovoy, Vladimir ;

Ferns, Michael J. .

HUMAN GENETICS, 2012, 131 (07) :1123-1135

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