The search for new antigenic targets in myasthenia gravis

被引：63

作者：

Cossins, Judith ^{[1
]}

Belaya, Katsiaryna ^{[1
]}

Zoltowska, Katarzyna ^{[1
]}

Koneczny, Inga ^{[1
]}

Maxwell, Susan ^{[1
]}

Jacobson, Leslie ^{[2
]}

Leite, Maria Isabel ^{[2
]}

Waters, Patrick ^{[2
]}

Vincent, Angela ^{[2
]}

Beeson, David ^{[1
]}

机构：

[1] John Radcliffe Hosp, Weatherall Inst Mol Med, Nuffield Dept Clin Neurosci, Oxford OX3 9DS, England

[2] John Radcliffe Hosp, Nuffield Dept Clin Neurosci, Oxford OX3 9DS, England

来源：

MYASTHENIA GRAVIS AND RELATED DISORDERS II | 2012年 / 1275卷

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

myasthenia; autoantibodies; AChR; Lrp4; agrin; ColQ; MuSK; ACETYLCHOLINE-RECEPTOR; NEUROMUSCULAR-JUNCTION; COLQ MUTATION; KINASE MUSK; PROTEIN; ANTIBODIES; AUTOANTIBODIES; AGRIN; LRP4; GENE;

D O I：

10.1111/j.1749-6632.2012.06833.x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Around 80% of myasthenia gravis patients have antibodies against the acetylcholine receptor, and 0-60% of the remaining patients have antibodies against the muscle-specific tyrosine kinase, MuSK. Another recently identified antigen is low-density lipoprotein receptor-related protein 4 (Lrp4). To improve the existing assays and widen the search for new antigenic targets, we have employed cell-based assays in which candidate target proteins are expressed on the cell surface of transfected cells and probed with patient sera. These assays, combined with use of myotube cultures to explore the effects of the antibodies, enable us to begin to identify new antigenic targets and test antibody pathogenicity in vitro.

引用

页码：123 / 128

页数：6

共 38 条

[1] A MYASTHENIA-GRAVIS PLASMA IMMUNOGLOBULIN REDUCES MINIATURE ENDPLATE POTENTIALS AT HUMAN ENDPLATES INVITRO [J].

BURGES, J ;

WRAY, DW ;

PIZZIGHELLA, S ;

HALL, Z ;

VINCENT, A .

MUSCLE & NERVE, 1990, 13 (05) :407-413

[2] MuSK is required for anchoring acetylcholinesterase at the neuromuscular junction [J].

Cartaud, A ;

Strochlic, L ;

Guerra, M ;

Blanchard, B ;

Lambergeon, M ;

Krejci, E ;

Cartaud, J ;

Legay, C .

JOURNAL OF CELL BIOLOGY, 2004, 165 (04) :505-515

[3] Mutation in the human acetylcholinesterase-associated collagen gene, COLQ, is responsible for congenital myasthenic syndrome with end-plate acetylcholinesterase deficiency (type Ic) [J].

Donger, C ;

Krejci, E ;

Serradell, AP ;

Eymard, B ;

Bon, S ;

Nicole, S ;

Chateau, D ;

Gary, F ;

Fardeau, M ;

Massoulié, J ;

Guicheney, P .

AMERICAN JOURNAL OF HUMAN GENETICS, 1998, 63 (04) :967-975

[4] MYASTHENIA-GRAVIS AND MYASTHENIC SYNDROMES [J].

ENGEL, AG .

ANNALS OF NEUROLOGY, 1984, 16 (05) :519-534

[5] Effect of sera from AChR-antibody negative myasthenia gravis patients on AChR and MuSK in cell cultures [J].

Farrugia, Maria Elena ;

Bonifati, Domenico Marco ;

Clover, Linda ;

Cossins, Judy ;

Beeson, David ;

Vincent, Angela .

JOURNAL OF NEUROIMMUNOLOGY, 2007, 185 (1-2) :136-144

[6] ACETYLCHOLINE RECEPTOR-AGGREGATING ACTIVITY OF AGRIN ISOFORMS AND MAPPING OF THE ACTIVE-SITE [J].

GESEMANN, M ;

DENZER, AJ ;

RUEGG, MA .

JOURNAL OF CELL BIOLOGY, 1995, 128 (04) :625-636

[7] The Extracellular Region of Lrp4 Is Sufficient to Mediate Neuromuscular Synapse Formation [J].

Gomez, Andrea M. ;

Burden, Steven J. .

DEVELOPMENTAL DYNAMICS, 2011, 240 (12) :2626-2633

[8] Recurrent COLQ Mutation in Congenital Myasthenic Syndrome [J].

Guven, Alev ;

Demirci, Mehmet ;

Anlar, Banu .

PEDIATRIC NEUROLOGY, 2012, 46 (04) :253-256

[9] MODULATION OF ACETYLCHOLINE-RECEPTOR BY ANTIBODY AGAINST RECEPTOR [J].

HEINEMANN, S ;

BEVAN, S ;

KULLBERG, R ;

LINDSTROM, J ;

RICE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (07) :3090-3094

[10] Autoantibodies to Low-Density Lipoprotein Receptor-Related Protein 4 in Myasthenia Gravis [J].

Higuchi, Osamu ;

Hamuro, Johko ;

Motomura, Masakatsu ;

Yamanashi, Yuji .

ANNALS OF NEUROLOGY, 2011, 69 (02) :418-422

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