Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage

被引：14

作者：

Zheng, Jun ^{[1
,2
]}

Gong, Pengtao ^{[1
]}

Jia, Honglin ^{[2
]}

Li, Mingying ^{[1
]}

Zhang, Guocai ^{[1
]}

Zhang, Xichen ^{[1
]}

Li, Jianhua ^{[1
]}

机构：

[1] Jilin Univ, Coll Vet Med, Minist Educ, Key Lab Zoonosis, Changchun 130023, Peoples R China

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin, Peoples R China

来源：

VETERINARY PARASITOLOGY | 2014年 / 201卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Eimeria tenella; Rhomboid; Microneme; Interaction; INVASION; TOXOPLASMA;

D O I：

10.1016/j.vetpar.2014.01.010

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Invasion in several apicomplexan parasites, including Eimeria tenella, is accompanied by shedding of surface adhesins by intramembrane proteolysis mediated by rhomboid protease. We have previously identified E. tenella rhomboid 3 (EtROM3), but its precise role has not been elucidated. In this study, the interactions between EtROM3 and microneme (MIC) proteins were analyzed using the yeast two hybrid technique. The results showed that c-Myc-ROM3 fusion protein interacted with EtMIC4 protein in co-transformed AH109 yeasts, which was further confirmed by immunoprecipitation assay. Smaller EtMIC4 band from co-transformed cells suggested that EtROM3 was an active protease and involved in the cleavage of EtMIC4. (C) 2014 The Authors. Published by Elsevier B.V. All rights reserved.

引用

页码：146 / 149

页数：4

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