TGF-β1 mimics the effect of IL-4 on the glycosylation of IgA1 by downregulating core 1 β1, 3-galactosyltransferase and Cosmc

被引：17

作者：

Xiao, Jun ^{[1
]}

Wang, Manting ^{[2
]}

Xiong, Dawei ^{[3
]}

Wang, Ying ^{[1
]}

Li, Qiuyue ^{[1
]}

Zhou, Jing ^{[1
]}

Chen, Qinkai ^{[1
]}

机构：

[1] Nanchang Univ, Affiliated Hosp 1, Dept Nephrol, 17 YongWai St, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Univ, Affiliated Jiujiang Hosp, Dept Nephrol, Jiujiang 332000, Jiangxi, Peoples R China

[3] Jiangxi Acad Sci, Inst Microbiol, Nanchang 330096, Jiangxi, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 15卷 / 02期

关键词：

IgA nephropathy; transforming growth factor-beta 1; IgA1; glycosylation; core1; beta; 1; 3-galactosyltransferase; Cosmc; IMMUNOGLOBULIN-A NEPHROPATHY; ABERRANT O-GLYCOSYLATION; TRANSFORMING GROWTH-FACTOR-BETA-1; MASS-SPECTROMETRY; T-CELLS; PATHOGENESIS; EXPRESSION; BETA-1,3-GALACTOSYLTRANSFERASE; ACTIVATION; CYTOKINES;

D O I：

10.3892/mmr.2016.6084

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aberrant glycosylation of IgA1 is pivotal in the pathogenesis of IgA nephropathy (IgAN). The aim of the present study was to investigate the effect of transforming growth factor-beta 1 (TGF-beta 1) on the glycosylation of IgA1 and the associated mechanism. The mRNA levels of core1 beta 1, 3-galactosyltransferase (C1GalT1) and its molecular chaperone, Cosmc, were analyzed, as was the subsequent O-glycosylation of IgA1, in a human B-cell line stimulated with TGF-beta 1. The IgA1-positive human B-cell line was cultured with different concentrations of recombinant human TGF-beta 1 (5, 10, 15 and 30 ng/ml). The production and glycosylation of IgA1 were assayed using sandwich ELISA and enzyme-linked lectin binding assays, respectively, and the mRNA levels of C1GalT1 and Cosmc were quantified using reverse transcription-quantitative polymerase chain reaction analysis. The results showed that the production of IgA1 was stimulated by low concentrations of TGF-beta 1 (5 or 10 ng/ml) and was suppressed by high concentrations (15 or 30 ng/ml). The terminal glycosylation of secreted IgA1 was altered in response to TGF-beta 1. TGF-beta 1 stimulation significantly decreased the mRNA levels of C1GalT1 and Cosmc. TGF-beta 1 may be key in controlling the glycosylation of IgA1, in part via the downregulation of C1GalT1 and Cosmc.

引用

页码：969 / 974

页数：6

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