Use of multilocus sequence typing and pulsed-field gel electrophoresis for the study of serogroup B Neisseria meningitidis isolates from Casablanca (Morocco)

被引：2
作者：
Zerouali, K
Castelli, P
Van Looveren, M
El Mdaghri, N
Boudouma, M
Benbachir, M
Nicolas, P
机构：
[1] Fac Med Casablanca, Microbiol Lab, Casablanca 20000, Morocco
[2] IMTSSA Ctr Collaborateur OMS, Unite Meningocoque, Marseille, France
[3] UA, Univ Hosp Antwerp, Dept Med Microbiol, Antwerp, Belgium
[4] Fac Sci Ben Msik, Dept Biol, Casablanca, Morocco
来源：
PATHOLOGIE BIOLOGIE | 2006年 / 54卷 / 03期
关键词：
Neisseria meningitidis; serogroup B; MLST; PFGE; Casablanca; Morocco;
D O I：
10.1016/j.patbio.2005.09.002
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A previous study showed that B:4:P1.15 was the most frequent phenotype of Neisseria meningitidis isolated in Casablanca (Morocco). To determine if there was an epidemic clone, MLST and PFGE were used to compare 13 B:4:P1.15 strains isolated from September 1999 to December 2000. MLST showed 4 Sequence Types (ST): ST-33 was the most frequent ST (9/13 strains) and 4 strains belonged to 3 newly described STs. Twelve stains belonged to ST-32 complex, and one strain presenting a new ST (ST-2502) did not belong to any known ST complex. The analysis by PFGE showed that the strains were subdivided into 7 clusters, and that there was no epidemic clone. MLST is useful for long-term epidemiological studies on N. meningitidis strains from varied geographical origins. PFGE seemed to be well adapted to the comparison of a small number of strains isolated during a short period within a defined community. (c) 2005 Elsevier SAS. Tous droits reserves.
引用
收藏
页码：166 / 170
页数：5
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