Use of multilocus sequence typing and pulsed-field gel electrophoresis for the study of serogroup B Neisseria meningitidis isolates from Casablanca (Morocco)

被引：2
作者：
Zerouali, K
Castelli, P
Van Looveren, M
El Mdaghri, N
Boudouma, M
Benbachir, M
Nicolas, P
机构：
[1] Fac Med Casablanca, Microbiol Lab, Casablanca 20000, Morocco
[2] IMTSSA Ctr Collaborateur OMS, Unite Meningocoque, Marseille, France
[3] UA, Univ Hosp Antwerp, Dept Med Microbiol, Antwerp, Belgium
[4] Fac Sci Ben Msik, Dept Biol, Casablanca, Morocco
来源：
PATHOLOGIE BIOLOGIE | 2006年 / 54卷 / 03期
关键词：
Neisseria meningitidis; serogroup B; MLST; PFGE; Casablanca; Morocco;
D O I：
10.1016/j.patbio.2005.09.002
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A previous study showed that B:4:P1.15 was the most frequent phenotype of Neisseria meningitidis isolated in Casablanca (Morocco). To determine if there was an epidemic clone, MLST and PFGE were used to compare 13 B:4:P1.15 strains isolated from September 1999 to December 2000. MLST showed 4 Sequence Types (ST): ST-33 was the most frequent ST (9/13 strains) and 4 strains belonged to 3 newly described STs. Twelve stains belonged to ST-32 complex, and one strain presenting a new ST (ST-2502) did not belong to any known ST complex. The analysis by PFGE showed that the strains were subdivided into 7 clusters, and that there was no epidemic clone. MLST is useful for long-term epidemiological studies on N. meningitidis strains from varied geographical origins. PFGE seemed to be well adapted to the comparison of a small number of strains isolated during a short period within a defined community. (c) 2005 Elsevier SAS. Tous droits reserves.
引用
收藏
页码：166 / 170
页数：5
相关论文
共 46 条
[21]   Improved protocol for isolation of Campylobacter spp. from retail broiler meat and use of pulsed field gel electrophoresis for the typing of isolates [J].
Oyarzabal, Omar A. ;
Williams, Aretha ;
Zhou, Ping ;
Samadpour, Mansour .
JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 95 (01) :76-83
[22]   Comparison of IS1245 restriction fragment length polymorphism and pulsed-field gel electrophoresis for typing clinical isolates of Mycobacterium avium subsp avium [J].
Garriga, X ;
Cortés, P ;
Rodríguez, P ;
March, F ;
Prats, G ;
Coll, P .
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2000, 4 (05) :463-472
[23]   Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis [J].
Ido, Noriko ;
Iwabuchi, Kaori ;
Sato'o, Yusuke ;
Sato, Yasuo ;
Sugawara, Masaru ;
Yaegashi, Gakuji ;
Konno, Masaru ;
Akiba, Masato ;
Tanaka, Kiyoshi ;
Omoe, Katsuhiko ;
Uchida, Ikuo .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2015, 77 (05) :609-613
[24]   Molecular Typing of Japanese Field Isolates and Live Commercial Vaccine Strain of Mycoplasma synoviae Using Improved Pulsed-Field Gel Electrophoresis and vlhA Gene Sequencing [J].
Harada, Kazuki ;
Kijima-Tanaka, Mayumi ;
Uchiyama, Mariko ;
Yamamoto, Tomoko ;
Oishi, Koji ;
Arao, Megumi ;
Takahashi, Toshio .
AVIAN DISEASES, 2009, 53 (04) :538-543
[25]   Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in Sao Paulo, Brazil [J].
Barancelli, Giovana V. ;
Camargo, Tarsila M. ;
Gagliardi, Natalia G. ;
Porto, Ernani ;
Souza, Roberto A. ;
Campioni, Fabio ;
Falcao, Juliana P. ;
Hofer, Ernesto ;
Cruz, Adriano G. ;
Oliveira, Carlos A. F. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2014, 173 :21-29
[26]   Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India [J].
Yadav, Jay P. ;
Das, Suresh C. ;
Dhaka, Pankaj ;
Mukhopadhyay, Asish K. ;
Chowdhury, Goutam ;
Naskar, Syamal ;
Malik, Satyaveer S. .
INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE, 2018, 6 (01) :123-126
[27]   Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of Salmonella serotype Enteritidis, Typhimurium and Infantis isolates obtained from whole liquid eggs [J].
Rivoal, Katell ;
Protais, Jocelyne ;
Queguiner, Stephane ;
Boscher, Evelyne ;
Chidaine, Berengere ;
Rose, Valerie ;
Gautier, Michel ;
Baron, Florence ;
Grosset, Noel ;
Ermel, Gwennola ;
Salvat, Gilles .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 129 (02) :180-186
[28]   Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination of antibiotic resistance genes [J].
Millan Laplana, Leticia ;
Goni Cepero, M. Pilar ;
Ruiz, Joaquim ;
Zolezzi, Paula Cerda ;
Rubio Calvo, M. Carmen ;
Canales Erazo, Melisa ;
Gomez-Lus, Rafael .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 30 (06) :505-513
[29]   Molecular study of carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenems and determining their clonal relationship using pulsed-field gel electrophoresis [J].
Shahcheraghi, Fereshteh ;
Aslani, Mohammad Mehdi ;
Mahmoudi, Hassan ;
Karimitabar, Zahra ;
Solgi, Hamid ;
Bahador, Abbas ;
Alikhani, Mohammad Yousef .
JOURNAL OF MEDICAL MICROBIOLOGY, 2017, 66 (05) :570-576
[30]   Comparison of automated repetitive-sequence-based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus [J].
Church, Deirdre L. ;
Chow, Barbara L. ;
Lloyd, Tracie ;
Gregson, Daniel B. .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2011, 69 (01) :30-37
12345