High-Definition Differential Ion Mobility Spectrometry with Resolving Power up to 500

被引：69

作者：

Shvartsburg, Alexandre A. ^{[1
]}

Seim, Tom A. ^{[1
]}

Danielson, William F. ^{[1
]}

Norheim, Randy ^{[1
]}

Moore, Ronald J. ^{[1
]}

Anderson, Gordon A. ^{[1
]}

Smith, Richard D. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2013年 / 24卷 / 01期

关键词：

Ion mobility spectrometry; Differential IMS; FAIMS; High-resolution analyses; FLIGHT MASS-SPECTROMETER; ESI-FAIMS-MS; SEPARATION PROCESS; HISTONE TAILS; RESOLUTION; ANALYZERS; GASES; SYSTEM;

D O I：

10.1007/s13361-012-0517-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As the resolution of analytical methods improves, further progress tends to be increasingly limited by instrumental parameter instabilities that were previously inconsequential. This is now the case with differential ion mobility spectrometry (FAIMS), where fluctuations of the voltages and gas pressure have become critical. A new high-definition generator for FAIMS compensation voltage reported here provides a stable and accurate output than can be scanned with negligible steps. This reduces the spectral drift and peak width, thus improving the resolving power (R) and resolution. The gain for multiply-charged peptides that have narrowest peaks is up to similar to 40 %, and R similar to 400-500 is achievable using He/N-2 or H-2/N-2 gas mixtures.

引用

页码：109 / 114

页数：6

共 34 条

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