The effect of low-intensity pulsed ultrasound on repair of epithelial cell monolayers in vitro

被引：22
作者：
Hill, GE
Fenwick, S
Matthews, BJ
Chivers, RA
Southgate, J [1 ]
机构：
[1] Univ York, Dept Biol, Jack Birch Unit Mol Carcinogenesis, York YO10 5YW, N Yorkshire, England
[2] Smith & Nephew Res Ctr, York, N Yorkshire, England
[3] Biomat & Tissue Engn Ctr Ind Collaborat, Leeds, W Yorkshire, England
来源：
ULTRASOUND IN MEDICINE AND BIOLOGY | 2005年 / 31卷 / 12期
关键词：
wound healing; ultrasound; migration; proliferation; in vitro; re-epithelialization; epithelium; urothelium;
D O I：
10.1016/j.ultrasmedbio.2005.08.001
中图分类号：
O42 [声学];
学科分类号：
070206 ; 082403 ;
摘要：
Low intensity pulsed ultrasound (LIPUS) is widely used to accelerate tissue regeneration following injury, but the biological mechanisms of this effect are poorly understood. An in vitro model of epithelial wound healing was used to investigate the effect of LIPUS on the reepithelialization of scrape wounds in normal human urothelial (NHU) cell monolayers. The effects of clinical doses of ultrasound treatment on NHU cell growth and migration were investigated in cells grown under optimal conditions, without growth supplements and in media containing low vs. physiological calcium concentrations. No differences in cell growth or migration were observed. We conclude that there is no direct effect upon uro-epithelial regeneration by therapeutic ultrasound in vitro and suggest that any stimulation of epithelial wound repair in vivo may occur indirectly, for example by modulating the extracellular matrix composition and/or production of paracrine factors by the stroma. (c) 2005 World Federation for Ultrasound in Medicine & Biology.
引用
收藏
页码：1701 / 1706
页数：6
相关论文
共 31 条
  • [11] Accelerated healing of distal radial fractures with the use of specific, low-intensity ultrasound - A multicenter, prospective, randomized, double-blind, placebo-controlled study
    Kristiansen, TK
    Ryaby, JP
    McCabe, J
    Frey, JJ
    Roe, LR
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1997, 79A (07) : 961 - 973
  • [12] The effect of ultrasound on collagen synthesis and fibroblast proliferation in vitro
    Ramirez, A
    Schwane, JA
    McFarland, C
    Starcher, B
    [J]. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 1997, 29 (03) : 326 - 332
  • [13] The use of low-intensity ultrasound to accelerate the healing of fractures
    Rubin, C
    Bolander, M
    Ryaby, JP
    Hadjiargyrou, M
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2001, 83A (02) : 259 - 270
  • [14] Reconstitution of human urothelium from monolayer culture
    Scriven, SD
    Booth, C
    Thomas, DFM
    Trejdosiewicz, LK
    Southgate, J
    [J]. JOURNAL OF UROLOGY, 1997, 158 (03) : 1147 - 1152
  • [15] Host microenvironment in breast cancer development - Extracellular matrix-stromal cell contribution to neoplastic phenotype of epithelial cells in the breast
    Shekhar, MPV
    Pauley, R
    Heppner, G
    [J]. BREAST CANCER RESEARCH, 2003, 5 (03) : 130 - 135
  • [16] Smith BA, 2001, CANCER RES, V61, P1678
  • [17] SOUTHGATE J, 1994, LAB INVEST, V71, P583
  • [18] Southgate J, 1999, ADV EXP MED BIOL, V462, P19
  • [19] Southgate J., 2002, CULTURE HUMAN UROTHE, VSecond, P381, DOI DOI 10.1002/0471221201.CH12
  • [20] STEWART FA, 1986, BRIT J CANCER, V53, P280
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