Development of Immunochromatographic Test Strip based on Quantum Dot Nanoparticles (QDs)

被引：0

作者：

Sioloetwong, T. ^{[1
]}

Kopwitthaya, A. ^{[4
]}

Sathirapongsasuti, N. ^{[5
]}

Nawattanapaiboon, K. ^{[1
]}

Kitiyakara, C. ^{[6
]}

Srikhirin, T. ^{[1
,2
,3
]}

机构：

[1] Mahidol Univ, Sch Mat Sci & Innovat, Fac Sci, 999 Phutthamonthon Sai4 Rd, Salaya 73170, Nakhon Pathom, Thailand

[2] Mahidol Univ, Dept Phys, Fac Sci, 272 Rama 6 Rd, Khawaeng Thung Phaya Tha 10400, Ratchathewi Ban, Thailand

[3] Mahidol Univ, NANOTEC Ctr Excellence, Fac Sci, 272 Rama 6 Rd, Khawaeng Thung Phaya Tha 10400, Ratchathewi Ban, Thailand

[4] Natl Sci & Technol Dev Agcy, Natl Elect & Comp Technol Ctr, 111 Phaholyothin Rd, Khlong Luang 12120, Pathum Thani, Thailand

[5] Mahidol Univ, Fac Med, Dept Translat Med Ramathibodi, Ramathobodi Hosp, 270 Rama 6 Rd, Khawaeng Thung Phaya Tha 10400, Ratchathewi Ban, Thailand

[6] Mahidol Univ, Fac Med, Ramathobodi Hosp, Dept Med, 270 Rama 6 Rd, Khawaeng Thung Phaya Tha 10400, Ratchathewi Ban, Thailand

来源：

3RD INTERNATIONAL CONFERENCE ON MATERIALS AND INTELLIGENT MANUFACTURING (ICMIM) | 2019年 / 654卷

关键词：

D O I：

10.1088/1757-899X/654/1/012011

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Water-soluble fluorescent CdSe/CdZnS quantum dots (QDs) (lambda(em)=637nm) were used as a labelling reporter for strip detection. The CdSe/CdZnS QDs was prepared by using core shell technique where CdSe QDs (lambda(em)=590nm) core was capped with CdZnS shell. CdZnS-QDs were conjugated with amine-modified DNA probe, that can bio-recognize with DNA product for use in lateral flow assays (LFAs) detection. The presence of functionalized water-dispersible QDs was confirmed by Fourier transform infra-red (FTIR) technique, where a characteristic carboxylic group was observed at 1633 cm(-1) (-OH) and 3310 cm(-1) (-COO). DNA probe was immobilized onto QDs by EDC/NHS pathways and confirmed by protein absorption spectra at 260 nm due to heterocyclic ring of nucleosides. QDs-DNA can be applied as labelling reporter to readout fluorescence signal on the LFAs detection.

引用

页数：5

共 10 条

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