Fluorescence Intensity Fluctuations in Single Molecule Polarization Sensitive Measurements

被引：0

作者：

Turgeman, Lior ^{[1
,2
]}

Fixler, Dror ^{[1
,2
]}

机构：

[1] Bar Ilan Univ, Fac Engn, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Inst Nanotechnol & Adv Mat, IL-52900 Ramat Gan, Israel

来源：

SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING V | 2012年 / 8228卷

关键词：

Single molecule fluorescence intensity; Fluorescence intensity fluctuations; Molecule dipole reorientation; Brownian rotational diffusion; Monte-Carlo simulation; ORIENTATION; DIFFUSION; POLYMER;

D O I：

10.1117/12.907270

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A general framework to include fluctuations in the single molecule fluorescence intensity (FI) signal due to random changes in molecule dipole orientation was introduced at Optics Express (21, 2007). By assuming continuous changes in dipole orientation described by Brownian rotational diffusion, this research derives the probability density function (PDF) equation of FI fluctuations. Solution of the proposed equation for several limiting cases and different correlation times yields the short time behavior of FI fluctuations. Monte Carlo simulations results, in accordance with those found in theory will be presented during our talk.

引用

页数：13

共 9 条

[1] Three-dimensional single molecule rotational diffusion in glassy state polymer films
Bartko, AP
Xu, KW
Dickson, RM
[J]. PHYSICAL REVIEW LETTERS, 2002, 89 (02) : 261011 - 261014
[2] OPTICAL-SPECTRA AND KINETICS OF SINGLE IMPURITY MOLECULES IN A POLYMER - SPECTRAL DIFFUSION AND PERSISTENT SPECTRAL HOLE BURNING
BASCHE, T
AMBROSE, WP
MOERNER, WE
[J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 1992, 9 (05) : 829 - 836
[3] Determination of the orientational distribution and orientation factor for transfer between membrane-bound fluorophores using a confocal microscope
Corry, Ben
Jayatilaka, Dylan
Martinac, Boris
Rigby, Paul
[J]. BIOPHYSICAL JOURNAL, 2006, 91 (03) : 1032 - 1045
[4] Debye P., 1945, POLAR MOL
[5] Einstein A, 1905, ANN PHYS-BERLIN, V18, P639
[6] Photon statistics in single molecule orientational imaging
Foreman, Matthew R.
Sherif, S. Sherif
Toeroek, Peter
[J]. OPTICS EXPRESS, 2007, 15 (21): : 13597 - 13606
[7] Rapid determination of the three-dimensional orientation of single molecules
Fourkas, JT
[J]. OPTICS LETTERS, 2001, 26 (04) : 211 - 213
[8] Lakowicz J. R., 2006, PRINCIPLES FLUORESCE, P786
[9] Turgeman L., 2011, SHORT TIME BEH UNPUB

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