Utilizing Terminal Oxidants to Achieve P450-Catalyzed Oxidation of Methane

被引：38
作者：
Chen, Mike M. [1 ]
Coelho, Pedro S. [1 ]
Arnold, Frances H. [1 ]
机构：
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
来源：
ADVANCED SYNTHESIS & CATALYSIS | 2012年 / 354卷 / 06期
关键词：
alkanes; C?H activation; compound I; enzyme catalysis; metalloenzymes; methane oxidation; CYTOCHROME-P450; ENZYMES; MONOOXYGENASE; SUBSTRATE; P450; HYDROXYLATION; ACTIVATION; MECHANISM; SPIN; BIOTECHNOLOGY; BIOCATALYSTS;
D O I：
10.1002/adsc.201100833
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Terminal oxidant-supported P450 reactions alleviate the need for substrate binding to initiate catalysis by chemically generating compound I. This allows investigation of the innate substrate range of the enzyme active site. Using iodosylbenzene as the oxidant, CYP153A6, a medium-chain terminal alkane hydroxylase, exhibits methanol formation in the presence of methane demonstrating that P450-mediated methane hydroxylation is possible.
引用
收藏
页码：964 / 968
页数：5
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