Staining of antinuclear antibodies and antibodies against removable nuclear antigens in connective tissue diseases

被引：1

作者：

Oliva Menacho, Jose Enrique ^{[1
,2
]}

Luis Arroyo-Acevedo, Jorge ^{[3
,4
]}

Arturo Oliva-Candela, Jose ^{[5
]}

Antonio Garcia-Hjarles, Marco ^{[6
]}

Dominguez-Huarcaya, Lester ^{[3
,7
]}

机构：

[1] Cayetano Heredia Peruvian Univ, Fac Med, Lima, Peru

[2] Natl Major Univ San Marcos, Arzobispo Loayza Natl Hosp, Postgrad Unit, Fac Med, Lima, Peru

[3] Natl Univ San Marcos, Fac Med, Inst Clin Res, Lima, Peru

[4] Natl Univ San Marcos, Fac Med, Lab Expt Pharmacol, Lima, Peru

[5] Arzobispo Loayza Natl Hosp, Lima, Peru

[6] Cayetano Heredia Peruvian Univ, Fac Sci & Philosophy, Fac Med, Lima, Peru

[7] Univ Norbert Wiener, Fac Med, Lima, Peru

来源：

ALLERGOLOGIA ET IMMUNOPATHOLOGIA | 2020年 / 48卷 / 01期

关键词：

Antibodies against extractable nuclear antigens; Anti-Nuclear antibody staining pattern; Connective tissue diseases; Immunoblot; Indirect immunofluorescence;

D O I：

10.1016/j.aller.2019.07.002

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Introduction and objectives: Connective tissue diseases are inflammatory, autoimmune diseases and threaten quality of life. To determine the relationship between staining patterns of antinuclear antibodies and antibodies against extractable nuclear antigens in patients with connective tissue disease. Materials and methods: Observational, basic, analytical and transversal study. Study conducted in the Immunology Service of the Arzobispo Loayza National Hospital between January 2017 and June 2017. We analyzed 291 samples of patients with CTD and for the detection of antinuclear antibody staining patterns, the immunological kit and observation with microscope of at 40X Immunofluorescence and for the detection of the antibodies against extractable nuclear antigens. The Immunoblot method was employed. Statistical analyses were carried out with the statistical package SPSS version 21 for Windows. We used the Pearson Chi-square test for the categorical variables, a value of p < 0.05 was considered significant. Results: There was a significant relationship p < 0.05 of the homogeneous pattern, the mottled pattern with Anti-histones (p = 0.000), Anti-nucleosomes (p = 0.000), Anti-Ro 52 (p = 0.000), Anti SSA (p = 0.001), Anti-SSB (p = 0.003), Anti-dsDNA (p = 0.000) with the Pearson Chi-square test. There was a significant relationship of p < 0.05 of the centromeric pattern with Anti-Cenp B (p = 0.000) with Fisher square s exact statistic. Conclusions: There was a significant relationship between the anti-nuclear antibody staining patterns and the antibodies to the core extractable antigens in patients with systemic lupus erythematosus, Sjogren square s syndrome, Calcinosis, Raynaud square s phenomenon, esophageal Dysmotility, sclerodactyly and Telangiectasia (CREST), Scleroderma and Polymyositis. (C) 2019 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.

引用

页码：18 / 25

页数：8

共 29 条

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