Control Efficiency of Biochar Loaded with Bacillus subtilis Tpb55 against Tobacco Black Shank

Black shank caused by Phytophthora nicotianae has become a destructive soil-borne disease to different flue-cured tobacco cultivars in Southwest China. The use of biochar amendments for microorganism synergy is a promising effective strategy for P. nicotianae development control. In this study, biochar samples were prepared from tamarisk with different pyrolization temperatures (300-500 degrees C). The effect of pyrolytic temperatures on the bacteria immobilization efficiency of biochar was investigated. B. subtilis Tpb55 was successfully loaded on different biochars as biocontrol composites. The survival investigation of the inoculum suggested that biochar pyrolized at 300 degrees C (BC300), with a large pore opening diameter; a greater pore volume exhibited a better Tpb55 immobilization. A pot experiment indicated that Tpb55-loaded BC300 had a more pronounced decrease in the disease severity index of black shank disease and an increase in the soil pH, alkali-hydrolyzable nitrogen, soil-available phosphorus, and available potassium. BC300 inoculated with Tpb55 showed the highest control effect (79.60%) against tobacco black shank in the pot experiments, with the lowest copy number of P. nicotianae DNA. In conclusion, biochar-immobilized Tpb55 may provide a new strategy for preventing and controlling tobacco black shank.