Choosing dyes for cw-STED nanoscopy using self-assembled nanorulers

被引：29

作者：

Beater, Susanne ^{[1
]}

Holzmeister, Phil ^{[1
]}

Pibiri, Enrico ^{[1
]}

Lalkens, Birka ^{[1
]}

Tinnefeld, Philip ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Phys & Theoret Chem, NanoBioSci Grp, D-38106 Braunschweig, Germany

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2014年 / 16卷 / 15期

基金：

欧洲研究理事会;

关键词：

FIELD OPTICAL NANOSCOPY; DNA ORIGAMI; SUPERRESOLUTION MICROSCOPY; SUBDIFFRACTION-RESOLUTION; FLUORESCENCE MICROSCOPY; STIMULATED-EMISSION; PATTERNS; STANDARDS; SHAPES; LIMIT;

D O I：

10.1039/c4cp00127c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Superresolution microscopy is currently revolutionizing optical imaging. A key factor for getting images of highest quality is - besides a well-performing imaging system - the labeling of the sample. We compared the fluorescent dyes Abberior Star 488, Alexa 488, Chromeo 488 and Oregon Green 488 for use in continuous wave (cw-)STED microscopy in aqueous buffer and in a durable polymer matrix. To optimize comparability, we designed DNA origami standards labeled with the fluorescent dyes including a bead-like DNA origami with dyes focused on one spot and a DNA origami with two marks at a designed distance of similar to 100 nm. Our data show that all dyes are well suited for cw-STED microscopy but that the optimal dye depends on the embedding medium. The precise comparison enabled by DNA origami nanorulers indicates that these structures have matured from the proof-of-concept to easily applicable tools in fluorescence microscopy.

引用

页码：6990 / 6996

页数：7

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