Atmospheric pressure, nonthermal plasma inactivation of MS2 bacteriophage: effect of oxygen concentration on virucidal activity

被引：52

作者：

Alshraiedeh, N. H. ^{[1
]}

Alkawareek, M. Y. ^{[1
]}

Gorman, S. P. ^{[1
]}

Graham, W. G. ^{[2
]}

Gilmore, B. F. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Pharm, Belfast BT9 7BL, Antrim, North Ireland

[2] Queens Univ Belfast, Ctr Plasma Phys, Belfast BT9 7BL, Antrim, North Ireland

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2013年 / 115卷 / 06期

关键词：

atmospheric pressure plasma; disinfection; MS2; bacteriophage; nonthermal plasma; norovirus; plasma sterilization; HUMAN NOROVIRUS; GASTROENTERITIS; STERILIZATION; DISINFECTANTS; SURROGATES; MECHANISMS; MODEL;

D O I：

10.1111/jam.12331

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

AimsThe main aim of this study was to determine the virucidal inactivation efficacy of an in-house-designed atmospheric pressure, nonthermal plasma jet operated at varying helium/oxygen feed gas concentrations against MS2 bacteriophage, widely employed as a convenient surrogate for human norovirus. Methods and ResultsThe effect of variation of percentage oxygen concentration in the helium (He) carrier gas was studied and found to positively correlate with MS2 inactivation rate, indicating a role for reactive oxygen species (ROS) in viral inactivation. The inactivation rate constant increased with increasing oxygen concentrations up to 075% O-2. 3 log(10) (999%) reductions in MS2 viability were achieved after 3min of exposure to the plasma source operated in a helium/oxygen (9925%:075%) gas mixture, with >7 log(10) reduction after 9min exposure. ConclusionsAtmospheric pressure, nonthermal plasmas may have utility in the rapid disinfection of virally contaminated surfaces for infection control applications. Significance and Impact of StudyThe atmospheric pressure, nonthermal plasma jet employed in this study exhibits rapid virucidal activity against a norovirus surrogate virus, the MS2 bacteriophage, which is superior to previously published inactivation rates for chemical disinfectants.

引用

页码：1420 / 1426

页数：7

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