Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins

被引：46
作者：
Altindis, Emrah [1 ]
Tefon, Burcu E. [1 ]
Yildirim, Volkan [1 ]
Ozcengiz, Erkan [2 ]
Becher, Doete [3 ]
Hecker, Michael [3 ]
Ozcengiz, Gulay [1 ]
机构：
[1] Middle E Tech Univ, Dept Biol Sci, TR-06531 Ankara, Turkey
[2] Vaccine Biol Res Co, Ankara, Turkey
[3] Ernst Moritz Arndt Univ Greifswald, Inst Mikrobiol, D-17487 Greifswald, Germany
来源：
VACCINE | 2009年 / 27卷 / 04期
关键词：
Bordetella pertussis; Immunoproteomics; Vaccine candidates; HEAT-SHOCK-PROTEIN; GEL-ELECTROPHORESIS; MYCOBACTERIUM-TUBERCULOSIS; STAPHYLOCOCCUS-EPIDERMIDIS; PSEUDOMONAS-AERUGINOSA; MOLECULAR PATHOGENESIS; CAMPYLOBACTER-JEJUNI; VACCINE CANDIDATES; MASS-SPECTROMETRY; SURFACE PROTEOME;
D O I：
10.1016/j.vaccine.2008.11.020
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：542 / 548
页数：7
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