Novel horseradish peroxidase substrates for use in immunohistochemistry

被引:13
作者
Petersen, Kenneth H. [1 ]
机构
[1] Dako AS, DK-2600 Glostrup, Denmark
关键词
Cyanine dye; Chromogen; Horseradish peroxidase; Immunohistochemistry; Double stain; HISTOCHEMISTRY; PRECIPITATION; AMPLIFICATION;
D O I
10.1016/j.jim.2008.09.018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
New chromogens expand the colour palette for horseradish peroxidase chromogens used in immunohistochemistry. Tissue staining of cytokeratin with three new cyanine-based chromogens is described. Their use as fluorescent reporters is demonstrated. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:86 / 89
页数:4
相关论文
共 10 条
[1]   COLOR MODIFICATION OF DIAMINOBENZIDINE (DAB) PRECIPITATION BY METALLIC-IONS AND ITS APPLICATION FOR DOUBLE IMMUNOHISTOCHEMISTRY [J].
HSU, SM ;
SOBAN, E .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (10) :1079-1082
[2]  
Krieg R, 2000, CELL MOL BIOL, V46, P1191
[3]   N,N-Dialkylaminostyryl dyes:: specific and highly fluorescent substrates of peroxidase and their application in histochemistry [J].
Krieg, R. ;
Eitner, A. ;
Guenther, W. ;
Schuerer, C. ;
Lindenau, J. ;
Halbhuber, K. -J. .
JOURNAL OF MOLECULAR HISTOLOGY, 2008, 39 (02) :169-191
[4]   Optimization of heterocyclic 4-hydroxystyryl derivatives for histological localization of endogenous and immunobound peroxidase activity [J].
Krieg, R. ;
Eitner, A. ;
Guenther, W. ;
Halbhuber, K.-J. .
BIOTECHNIC & HISTOCHEMISTRY, 2007, 82 (4-5) :235-262
[5]  
Krieg R, 2003, CELL MOL BIOL, V49, P547
[6]   CYANINE DYE LABELING REAGENTS CONTAINING ISOTHIOCYANATE GROUPS [J].
MUJUMDAR, RB ;
ERNST, LA ;
MUJUMDAR, SR ;
WAGGONER, AS .
CYTOMETRY, 1989, 10 (01) :11-19
[8]   Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide [J].
Schmidt, BF ;
Chao, J ;
Zhu, ZG ;
DeBiasio, RL ;
Fisher, G .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1997, 45 (03) :365-373
[9]   SEARCHING FOR PHOTOCHROMIC LIQUID-CRYSTALS SPIRONAPHTHOXAZINE SUBSTITUTED WITH A MESOGENIC GROUP [J].
SHRAGINA, L ;
BUCHHOLTZ, F ;
YITZCHAIK, S ;
KRONGAUZ, V .
LIQUID CRYSTALS, 1990, 7 (05) :643-655
[10]   Amplification methods to increase the sensitivity of in situ hybridization: Play CARD(S) [J].
Speel, EJM ;
Hopman, AHN ;
Komminoth, P .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1999, 47 (03) :281-288