Lectin as a marker for staining and purification of embryonic pancreatic epithelium

被引：27

作者：

Kobayashi, H ^{[1
]}

Spilde, TL ^{[1
]}

Li, ZX ^{[1
]}

Marosky, JK ^{[1
]}

Bhatia, AM ^{[1
]}

Hembree, MJ ^{[1
]}

Prasadan, K ^{[1
]}

Preuett, BL ^{[1
]}

Gittes, GK ^{[1
]}

机构：

[1] Childrens Mercy Hosp, Lab Surg Organogenesis, Kansas City, MO 64108 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 02期

关键词：

lectin; pancreas; differentiation; epithelium; purification; immunomagnetic separation;

D O I：

10.1016/S0006-291X(02)00278-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The embryonic pancreatic epithelium, and later the ductal epithelium, is known to give rise to the endocrine and exocrine cells of the developing pancreas, but no specific surface marker for these cells has been identified. Here, we utilized Dolichos Biflorus Agglutinin (DBA) as a specific marker of these epithelial cells in developing mouse pancreas. From the results of an immunofluorescence study using fluorescein-DBA and pancreatic specific cell markers, we found that DBA detects specifically epithelial, but neither differentiating endocrine cells nor acinar cells. We further applied this marker in an immunomagnetic separation system (Dynabead system) to purify these putative multi-potential cells from a mixed developing pancreatic cell population. This procedure could be applied to study differentiation and cell lineage selections in the developing pancreas, and also may be applicable to selecting pancreatic precursor cells for potential cellular engineering. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：691 / 697

页数：7

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