Aquitalea aquatilis sp. nov., isolated from Jungwon waterfall

A Gram-stain-negative, facultative anaerobic, motile, short rods and yellow-pigmented bacterium, designated strain THG-DN7.12(T), was isolated from water collected at Jungwon waterfall on Yongmun mountain. Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN7.12(T) was found to be most closely related to Aquitalea denitrificans 5YN1-3(T) (98.9% sequence similarity), Aquitalea magnusoniiTRO-001DR8(T) (98.7%) and Aquitalea pelogenes P1297(T) (98.0%). The DNA-DNA relatedness between strain THG-DN7.12T and its phylogenetically closest neighbours was below 70.0%. The strain's DNA G+C content was 59.7mol%. The major polar lipid was found to be phosphatidylethanolamine. Summed feature 3 (C-16(:1)omega 7c and/or C-16(:1)omega 6c) and C(16:0 )were identified as the major fatty acids. Ubiquinone 0-8 was detected as the only respiratory quinone. These data supported the affiliation of strain THG-DN7.12(T) to the genus Aquitalea. Strain THG-DN7.12(T) was distinguished from related Aquitalea species by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Aquitalea aquatilis sp. nov. is proposed, with THG-DN7.(12T )as the type strain (=KACC 18847(T)=CCTCC AB 2016185(T)).