Identifying evidence for hunter-gatherers in the palaeoenvironmental record is far from simple. Despite decades of research, few studies have demonstrated unambiguous palynological evidence of hunter-gatherers. This paper presents the results of high-resolution palaeoecological analyses of a peat sequence located within the vicinity of the pre-historic Yup'ik village of Nunalleq in southwestern Alaska. The aim of this research was to examine whether there are any discernible palaeoenvironmental impacts associated with the 15th-17th century occupation of the site. Presuming an ephemeral character to any palaeoecological signal, this study selected a sampling location approximately 30 m east of the limit of archaeological remains. Pollen, non-pollen palynomorphs and microscopic charcoal analysis were then used to generate a highly resolved (contiguous 1 cm) environmental history for the site. The results are striking and indicate that the activities of prehistoric Yup'ik hunter-gatherers at Nunalleq did leave a clear material trace in the palaeoenvironmental record. Through the application of high-resolution Pb-210 and C-14 dating and Bayesian modelling, these impacts were found to be concurrent with the occupation of the archaeological site. These findings suggest that not only can circumpolar hunter-gatherers leave a material palaeoenvironmental trace but that these traces may be used to accurately date such site activity in lieu of excavation.