Prophylactic effect of a peptide vaccine targeting TM4SF5 against colon cancer in a mouse model

被引:11
作者
Kwon, Sanghoon [1 ]
Kim, Young-Eun [2 ]
Kim, Dongbum [1 ]
Park, Byoung Kwon [3 ]
Wu, Guang [1 ]
Kim, Te Ha [3 ]
Choi, Song Hee [3 ]
Kim, Doo-Sik [4 ]
Kwon, Hyung-Joo [1 ,3 ]
Lee, Younghee [2 ]
机构
[1] Hallym Univ, Coll Med, Ctr Med Sci Res, Gangwon Do 200702, South Korea
[2] Chungbuk Natl Univ, Dept Biochem, Coll Nat Sci, Chungbuk 361763, South Korea
[3] Hallym Univ, Coll Med, Dept Microbiol, Gangwon Do 200702, South Korea
[4] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea
基金
新加坡国家研究基金会;
关键词
TM4SF5; Peptide vaccine; Antibody; Colon cancer; Prophylaxis; DNA-LIPOSOME COMPLEX; COLORECTAL-CANCER; CPG-DNA; MICROSATELLITE INSTABILITY; CARCINOMA; HEPATOCYTES; ACTIVATION; EXPRESSION; ANTIBODY; RECEPTOR;
D O I
10.1016/j.bbrc.2013.04.057
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Expression of transmembrane 4 superfamily member 5 protein (TM4SF5) was implicated in hepatocellular carcinoma (HCC) and colon cancer. Previously, we have shown that immunization with TM4SF5 peptide-CpG-DNA-liposome complex induces production of TM4SF5-specific antibodies and protects mice from HCC progression in an allograft model. Here, we confirmed expression of TM4SF5 in the mouse colon cancer cell line CT-26 and found that anti-TM4SF5 antibody inhibits growth of CT-26 cells. We then immunized mice with TM4SF5 peptide-CpG-DNA-liposome complex and transplanted CT-26 cells to investigate the vaccination effects. Robust production of TM4SF5-specific antibodies was induced by challenge with CT-26 cells and the tumor growth was significantly suppressed in the immunized mice. The peptide vaccine targeting TM4SF5 consequently showed a prophylactic effect against colon cancer development in a mouse model. These results suggest that the peptide vaccine can be potentially applied in humans to treat colon cancer. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:134 / 139
页数:6
相关论文
共 30 条
[1]  
Abbas A.K., 2007, CELLULAR MOL IMMUNOL
[2]   The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration [J].
Anderson, Keith R. ;
Singer, Ruth A. ;
Balderes, Dina A. ;
Hernandez-Lagunas, Laura ;
Johnson, Christopher W. ;
Artinger, Kristin B. ;
Sussel, Lori .
DEVELOPMENT, 2011, 138 (15) :3213-3224
[3]   Molecular Profiling of Colon Tumors: The Search for Clinically Relevant Biomarkers of Progression, Prognosis, Therapeutics, and Predisposition [J].
Bacolod, Manny D. ;
Barany, Francis .
ANNALS OF SURGICAL ONCOLOGY, 2011, 18 (13) :3694-3700
[4]   Variation in TCF7L2 and increased risk of colon cancer -: The Atherosclerosis Risk in Communities (ARIC) Study [J].
Folsom, Aaron R. ;
Pankow, James S. ;
Peacock, James M. ;
Bielinski, Suzette J. ;
Heiss, Gerardo ;
Boerwinkle, Eric .
DIABETES CARE, 2008, 31 (05) :905-909
[5]  
Forrester K., 1987, NATURE, V327, P293
[6]   Metastatic Colorectal Cancer: From Improved Survival to Potential Cure [J].
Gallagher, David J. ;
Kemeny, Nancy .
ONCOLOGY, 2010, 78 (3-4) :237-248
[7]   Mutations in the Human naked cuticle Homolog NKD1 Found in Colorectal Cancer Alter Wnt/Dvl/β-Catenin Signaling [J].
Guo, Jianhui ;
Cagatay, Tolga ;
Zhou, Guangjin ;
Chan, Chih-Chiang ;
Blythe, Shelby ;
Suyama, Kaye ;
Zheng, Li ;
Pan, Kaifeng ;
Qian, Chiping ;
Hamelin, Richard ;
Thibodeau, Stephen N. ;
Klein, Peter S. ;
Wharton, Keith A., Jr. ;
Liu, Wanguo .
PLOS ONE, 2009, 4 (11)
[8]   Tetraspanin CD151 as a target for antibody-based cancer immunotherapy [J].
Haeuw, Jean-Francois ;
Goetsch, Liliane ;
Bailly, Christian ;
Corvaia, Nathalie .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2011, 39 :553-558
[9]   Novelty of Axin 2 and lack of Axin 1 gene mutation in colorectal cancer: a study in Kashmiri population [J].
Khan, Nighat P. ;
Pandith, Arshad A. ;
Hussain, Mahboob Ul ;
Yousuf, Adfar ;
Khan, Mosin S. ;
Wani, Khursheed A. ;
Mudassar, Syed .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2011, 355 (1-2) :149-155
[10]   Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA [J].
Kim, Dongbum ;
Kwon, Hyung-Joo ;
Lee, Younghee .
BMB REPORTS, 2011, 44 (09) :607-612