Production, Purification and Characterization of β-glucosidase from Bacillus subtilis strain PS Isolated from Sugarcane Bagasse

被引：0
作者：
Verma, A. K. [1 ]
Saini, Soniya [1 ]
Nishad, S. [1 ]
Kumar, Vinod [1 ]
Singh, Shalini [1 ]
Dubey, Ashutosh [1 ]
机构：
[1] Govind Ballabh Pant Univ Agr & Technol, Dept Biochem, Coll Basic Sci & Humanities, Pantnagar 263145, Uttar Pradesh, India
来源：
JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2013年 / 7卷 / 01期
关键词：
beta-Glucosidase production; Gel filteration; Carbon source; Bacillus subtilis; BIOCHEMICAL-CHARACTERIZATION; MUTANT;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effect of various carbon sources on extracellular beta-glucosidase (BGL) production and its catalytic activity with different substrate was analyzed in Bacillus subtilis strain PS isolated from sugarcane bagasse. The highest extracellular enzyme activity was obtained in minimal medium with supplement of glucose was higher 0.42 U/ml, compared to other carbon sources such as maltose, cellobiose, lactose and xylose. Extracellular BGL was partially purified to 18.04 fold with specific activity 17.32 U/mg using Sephadex G-75 gel filtration chromatography after dialysis of 70% ammonium sulfate precipitation. In gel filteration fractions, three peaks having beta-glucosidase activity and represents isozymes of beta-glucosidase with molecular weight of 193 kDa, 64 kDa and 42 kDa, respectively. Purified BGL showed catalytic activity with lactose, cellobiose, pNPG and mellibiose.
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页码：803 / 810
页数：8
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