New strategy for enhancing the therapeutic index of Fungizone

被引：7

作者：

Belkherroubi-Sari, L. ^{[1
]}

Adida, H. ^{[1
]}

Seghir, A. ^{[1
]}

Boucherit, Z. ^{[1
]}

Boucherit, K. ^{[1
]}

机构：

[1] Univ Tlemcen, Lab Antibiot Antifong Physicochim Synth & Activit, Tilimsen, Algeria

来源：

JOURNAL DE MYCOLOGIE MEDICALE | 2013年 / 23卷 / 01期

关键词：

ANTIBIOTIC AMPHOTERICIN-B; TOXICITY; AGGREGATION; RESISTANCE; EFFICACY; DORMANCY;

D O I：

10.1016/j.mycmed.2012.10.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to enhance the therapeutic index of Fungizone®. For this reason we have prepared stock solutions of Fungizone® under different conditions of pHs (from 4.8 to 10.8) and temperatures (unheated solutions and heated ones during 20min at 70°C). Then we have evaluated the effect of those antifungal solutions on human red blood cells coming from one healthy donor, as well as on the dormancy of Candida albicans strain. The obtained results show that after exposure to antifungal stock solution prepared at pH 5.4 and heated during 20min at 70°C, the rate of hemolysis was largely reduced (only 5% of hemolysis). On the other hand, it is noticed the yeast regrowth after 26h whatever is the temperature or the pH of the stock solution. However at pH 5.4, the growth of C. albicans is inhibited at 75% in comparison with the one obtained with the conventional Fungizone® after 30h of culture where we observed 100% of growth. So, stock solution of Fungizone® prepared at pH 5.4 and heated 20min at 70°C seems to be a good strategy for enhancing the therapeutic index of Fungizone®. © 2012 Elsevier Masson SAS.

引用

页码：3 / 7

页数：5

共 19 条

[1] Impact of the Administration of Liposomal Amphotericin B in Patients with Renal Function Impairment at Initiation of Treatment [J].

Alvarez-Lerma, F. ;

Mariscal, F. ;

Quin-Tana, E. ;

Rialp, G. ;

Diaz-Reganon, J. ;

San Jose, M. J. Perez ;

Alvarez-Sanchez, B. .

JOURNAL OF CHEMOTHERAPY, 2010, 22 (04) :285-287

[2]

Belkherroubi-Sari L, 2008, AFR J MICROBIOL RES, V2, P242

[3]

Belkherroubi-Sari L., 2011, Food and Nutrition Sciences, V2, P731, DOI 10.4236/fns.2011.27100

[4] ONE-SIDED ACTION OF AMPHOTERICIN-B ON CHOLESTEROL-CONTAINING MEMBRANES IS DETERMINED BY ITS SELF-ASSOCIATION IN THE MEDIUM [J].

BOLARD, J ;

LEGRAND, P ;

HEITZ, F ;

CYBULSKA, B .

BIOCHEMISTRY, 1991, 30 (23) :5707-5715

[5] Dormancy of Candida albicans cells in the presence of the polyene antibiotic amphotericin B:: simple demonstration by flow cytometry [J].

Boucherit, Zahia ;

Seksek, Olivier ;

Bolard, Jacques .

MEDICAL MYCOLOGY, 2007, 45 (06) :525-533

[6] Antifungal drug resistance to azoles and polyenes [J].

Canuto, MM ;

Gutierrez, F .

LANCET INFECTIOUS DISEASES, 2002, 2 (09) :550-563

[7] How can micelle systems be rebuilt by a heating process? [J].

da Silva-Filho, Miguel Adelino ;

Vieira da Silva Siqueira, Scheyla Daniela ;

Freire, Larissa Bandeira ;

de Araujo, Ivonete Batista ;

Gyselle de Holanda e Silva, Kattya ;

Medeiros, Aldo da Cunha ;

Araujo-Filho, Irami ;

de Oliveira, Anselmo Gomes ;

Tabosa do Egito, Eryvaldo Socrates .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :141-150

[8]

Espada R, 2008, J ANTIMICROB CHEMOTH, V61, P1

[9] Heat-induced superaggregation of amphotericin B reduces its in vitro toxicity: a new way to improve its therapeutic index [J].

Gaboriau, F ;

Cheron, M ;

Petit, C ;

Bolard, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (11) :2345-2351

[10] Anomalously high aggregation level of the polyene antibiotic amphotericin B in acidic medium: Implications for the biological action [J].

Gagos, Mariusz ;

Herec, Monika ;

Arczewska, Marta ;

Czernel, Grzegorz ;

Dalla Serra, Mauro ;

Gruszecki, Wieslaw I. .

BIOPHYSICAL CHEMISTRY, 2008, 136 (01) :44-49

← 1 2 →