On the embryonic origin of adult melanophores: the role of ErbB and Kit signalling in establishing melanophore stem cells in zebrafish

被引:111
作者
Dooley, Christopher M. [1 ,2 ]
Mongera, Alessandro [1 ]
Walderich, Brigitte [1 ]
Nuesslein-Volhard, Christiane [1 ]
机构
[1] Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany
[2] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England
来源
DEVELOPMENT | 2013年 / 140卷 / 05期
关键词
Kit ligand a; ErbB; Dorsal root ganglia; Melanophore stem cells; NEURAL CREST DEVELOPMENT; PIGMENT PATTERN; C-KIT; MIGRATION; MELANOCYTES; MUTATIONS; SPECIFICATION; SURVIVAL; ENCODES; SOX10;
D O I
10.1242/dev.087007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pigment cells in vertebrates are derived from the neural crest (NC), a pluripotent and migratory embryonic cell population. In fishes, larval melanophores develop during embryogenesis directly from NC cells migrating along dorsolateral and ventromedial paths. The embryonic origin of the melanophores that emerge during juvenile development in the skin to contribute to the striking colour patterns of adult fishes remains elusive. We have identified a small set of melanophore progenitor cells (MPs) in the zebrafish (Danio rerio, Cyprinidae) that is established within the first 2 days of embryonic development in close association with the segmentally reiterated dorsal root ganglia (DRGs). Lineage analysis and 4D in vivo imaging indicate that progeny of these embryonic MPs spread segmentally, giving rise to the melanophores that create the adult melanophore stripes. Upon depletion of larval melanophores by morpholino knockdown of Mitfa, the embryonic MPs are prematurely activated; their progeny migrate along the spinal nerves restoring the larval pattern and giving rise to postembryonic MPs associated with the spinal nerves. Mutational or chemical inhibition of ErbB receptors blocks all early NC migration along the ventromedial path, causing a loss of DRGs and embryonic MPs. We show that the sparse like (slk) mutant lacks larval and metamorphic melanophores and identify kit ligand a (kitlga) as the underlying gene. Our data suggest that kitlga is required for the establishment or survival of embryonic MPs. We propose a model in which DRGs provide a niche for the stem cells of adult melanophores.
引用
收藏
页码:1003 / 1013
页数:11
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