An advanced uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) technique used in the sensitive and specific detection of Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in AIDS patients

被引：19

作者：

Fallahi, Shirzad ^{[1
,2
]}

Moosavi, Seyedeh Fatemeh ^{[2
]}

Karimi, Azadeh ^{[2
]}

Chegeni, Ali Sharafi ^{[3
]}

Saki, Mohammad ^{[3
]}

Namdari, Parsa ^{[4
]}

Rashno, Mohammad Menati ^{[5
]}

Varzi, Ali Mohamad ^{[6
]}

Tarrahi, Mohammad Javad ^{[7
]}

Almasian, Mohammad ^{[8
]}

机构：

[1] Lorestan Univ Med Sci, Razi Herbal Med Res Ctr, Khorramabad, Iran

[2] Lorestan Univ Med Sci, Fac Med, Dept Med Parasitol & Mycol, Khorramabad, Iran

[3] Lorestan Univ Med Sci, Deputy Hlth, Dept Communicable Dis Control & Prevent, Khorramabad, Iran

[4] Univ Debrecen, Fac Med, Debrecen, Hungary

[5] Islamic Azad Univ, Dept Biotechnol, Damghan Branch, Damghan, Iran

[6] Lorestan Univ Med Sci, Fac Med, Dept Immunol, Khorramabad, Iran

[7] Isfahan Univ Med Sci, Behav Sci Res Ctr, Dept Epidemiol & Stat, Esfahan, Iran

[8] Lorestan Univ Med Sci, Sch Med, Khorramabad, Iran

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 91卷 / 01期

关键词：

AIDS patients; Cryptosporidium spp; Detection; Rapid; Sensitive; UDG-LAMP; POLYMERASE-CHAIN-REACTION; WATERBORNE TRANSMISSION; PROTOZOAN PARASITES; TOXOPLASMA-GONDII; NESTED-PCR; OUTBREAK; INFECTION; SAMPLES; CONTAMINATION; PRODUCTS;

D O I：

10.1016/j.diagmicrobio.2017.12.017

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The rapid and accurate detection of Cryptosporidium spp. is critically important for the prevention and timely treatment of cryptosporidiosis in AIDS patients (APs). This study was conducted to examine a UDG-LAMP technique for the first time to diagnose cryptosporidiosis in APs. After collecting demographic and clinical data, three stool samples were collected from the participants (120 volunteering APs). The microscopic examination of stained smears using the acid-fast method and the UDG-LAMP assay were performed for each sample. 10% of APs were infected with Cryptosporidium spp. The number of detected cryptosporidiosis cases using the acid fast staining and UDG-LAMP methods were significantly different (P < 0.001). Diarrhea and weight loss were found to be significantly associated with cryptosporidiosis in patients (P < 0.05). The pretreatment of LAMP reagents with UDG successfully eliminated the likelihood of product re-amplification remaining from previous reactions. The UDG-LAMP technique could detect cryptosporidiosis in APs with high sensitivity and rapidity without carryover contamination. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：6 / 12

页数：7

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Arikan Sevtap, 1999, Acta Microbiologica et Immunologica Hungarica, V46, P33, DOI 10.1556/AMicr.46.1999.1.4

[2]

Badparva E., 2009, Pakistan Journal of Biological Sciences, V12, P1212, DOI 10.3923/pjbs.2009.1212.1216

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