Orofacial antinociceptive effect and antioxidant properties of the hydroethanol extract of Hyptis fruticosa salmz ex Benth

被引:22
作者
de Lima, Amanda C. B. [1 ]
Paixao, Monica S. [1 ]
Melo, Monica [1 ]
de Santana, Marilia T. [1 ]
Damascena, Nicole P. [1 ]
Dias, Antonio S. [1 ]
Porto, Yasmin C. B. S. [1 ]
Fernandes, Ximene A. [1 ]
Santos, Clisiane C. S. [1 ]
Lima, Clesio A. [1 ]
Quintans Junior, Lucindo J. [1 ]
Estevam, Charles dos S. [1 ]
Araujo, Brancilene S. [1 ]
机构
[1] Univ Fed Sergipe, Dept Fisiol, BR-49100000 Sao Cristovao, SE, Brazil
关键词
Hyptis fruticosa; Orofacial pain; Antioxidant activity; Lipoperoxidation; Oxygen reactive species; Antinociceptive activity; ESSENTIAL OIL; FORMALIN TEST; CAPSAICIN; MOUSE; MECHANISMS; GLUTAMATE; RECEPTOR; TARGETS; PAIN;
D O I
10.1016/j.jep.2012.12.031
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Hyptis fruticosa is a plant native to Brazil with antinociceptive and antiinflamatory properties. This study evaluated the antinociceptive activity of the hydroethanol extract of the plant leaves (CHEE) against orofacial pain as well as its in vitro effect against lipid peroxidation. Materials and methods: The antinociceptive activity was investigated in mice orally treated with different doses of the CHEE (50, 100, and 200 mg/kg) and morphine (5 mg/kg) using formalin, glutamate, and capsaicin orofacial pain models using. Lipoperoxidation was induced in egg yolk by AAPH and FeSO4 in the absence and presence of the CHEE (5, 50, 100, and 150 mu g/mL). Results: CHEE (200 mg/kg) significantly reduced (rho < 0.001) the pain response in the first (69.6%) and second (81.8%) phases of the formalin test, while the nociception caused by capsaicin was significantly (rho < 0.001) reduced by up to 62% at 200 mg/kg of extract. When glutamate was used as algogen; a significant (rho < 0.001) nociception reduction of up to 85% at 200 mg/kg extract was observed. CHEE showed a higher protection against lipoperoxidation caused by FeSO4 (82.3% TBARS inhibition) than AAPH (35.7% TBARS inhibition) at 150 mu g/mL. Conclusion: Hyptis fruticosa leaf CHEE is of pharmacological interest because it was able to inhibit the peripheral and central transmission of orofacial pain, while reducing the spreading of the inflammatory processes by neutralizing reactive oxygen species, which are by-products in the biosynthesis of pain mediators. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:192 / 197
页数:6
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