Oxidative DNA damage corresponds to the long term survival of human cells treated with silver nanoparticles

被引：52

作者：

Kruszewski, Marcin ^{[1
,2
]}

Gradzka, Iwona ^{[1
]}

Bartlomiejczyk, Teresa ^{[1
]}

Chwastowska, Jadwiga ^{[1
]}

Sommer, Sylwester ^{[1
]}

Grzelak, Agnieszka ^{[3
]}

Zuberek, Mariusz ^{[3
]}

Lankoff, Anna ^{[1
,4
]}

Dusinska, Maria ^{[5
]}

Wojewodzka, Maria ^{[1
]}

机构：

[1] Inst Nucl Chem & Technol, Ctr Radiobiol & Biol Dosimetry, PL-03195 Warsaw, Poland

[2] Inst Rural Hlth, Independent Lab Mol Biol, PL-20090 Lublin, Poland

[3] Univ Lodz, Dept Mol Biophys, PL-90131 Lodz, Poland

[4] Jan Kochanowski Univ, Inst Biol, Dept Radiobiol & Immunol, Kielce, Poland

[5] Norwegian Inst Air Res, Hlth Effects Grp, N-2027 Kjeller, Norway

来源：

TOXICOLOGY LETTERS | 2013年 / 219卷 / 02期

关键词：

Comet assay; gamma H2AX assay; Micronucleus assay; Clonogenic assay; Reactive oxygen species; COMET ASSAY; CELLULAR-RESPONSE; TOXICITY; SIZE; CYTOTOXICITY; GENOTOXICITY; PHOSPHORYLATION; APOPTOSIS; HISTONE; STATE;

D O I：

10.1016/j.toxlet.2013.03.006

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

We examined the relation between DNA damage and the clonogenic potential of 3 human cell lines, HepG2, HT29 and A549, treated with bare 20 nm or 200 nm silver nanoparticles (AgNPs). The endpoints examined were the DNA breakage estimated by the comet assay, the oxidative base damage recognized by formamido-pyrimidine glycosylase (FPG) and estimated with the FPG + comet assay, and the frequencies of histone gamma H2AX foci and micronuclei. Each cell line studied had a different pattern of DNA breakage and base damage versus the NPs concentration and time of treatment. The overall pattern of DNA breakage and base damage induction corresponded to the intracellular generation of reactive oxygen species. There was no increase in the frequencies of histone gamma H2AX foci and micronuclei as compared to those in the untreated cells. The reported experiments suggest that only the oxidative DNA damage corresponds to the loss of the clonogenic ability of cells treated with AgNPs. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：151 / 159

页数：9

共 43 条

[1] DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells [J].