Plasmonic hotspots of dynamically assembled nanoparticles in nanocapillaries: Towards a micro ribonucleic acid profiling platform

被引：13

作者：

Liu, Shoupeng ^{[1
]}

Yan, Yu ^{[1
]}

Wang, Yunshan ^{[1
]}

Senapati, Satyajyoti ^{[1
]}

Chang, Hsueh-Chia ^{[1
]}

机构：

[1] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA

来源：

BIOMICROFLUIDICS | 2013年 / 7卷 / 06期

基金：

美国国家科学基金会;

关键词：

FLUORESCENCE ENHANCEMENT; SILVER NANOPARTICLES; SPECTROSCOPY; RESONANCE;

D O I：

10.1063/1.4832095

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasmonic hot spots, generated by controlled 20-nm Au nanoparticle (NP) assembly, are shown to suppress fluorescent quenching effects of metal NPs, such that hair-pin FRET (Fluorescence resonance energy transfer) probes can achieve label-free ultra-sensitive quantification. The micron-sized assembly is a result of intense induced NP dipoles by focused electric fields through conic nanocapillaries. The efficient NP aggregate antenna and the voltage-tunable NP spacing for optimizing hot spot intensity endow ultra-sensitivity and large dynamic range (fM to pM). The large shear forces during assembly allow high selectivity (2-mismatch discrimination) and rapid detection (15 min) for a DNA mimic of microRNA. (C) 2013 AIP Publishing LLC.

引用

页数：4

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