Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

被引：11
作者：
Correddu, Danilo [1 ]
Aly, Sabrina Helmy
Di Nardo, Giovanna [1 ]
Catucci, Gianluca [1 ]
Prandi, Cristina [2 ]
Blangetti, Marco [2 ]
Bellomo, Chiara [2 ]
Bonometti, Elisabetta [2 ]
Viscardi, Guido [2 ]
Gilardi, Gianfranco [1 ]
机构：
[1] Univ Turin, Dept Life Sci & Syst Biol, Via Acad Albertina 13, I-10123 Turin, Italy
[2] Univ Turin, Dept Chem, Via P Giuria 7, I-10125 Turin, Italy
来源：
RSC ADVANCES | 2022年 / 12卷 / 52期
关键词：
CYTOCHROME-P450; BM3; LINALOOL; BIOTRANSFORMATION; MONOOXYGENASE; ASP251GLY/GLN307HIS; CHIMERAGENESIS; MONOTERPENES; DICLOFENAC; EXPRESSION; CYP102A1;
D O I：
10.1039/d2ra06029a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.
引用
收藏
页码：33964 / 33969
页数：6
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