Utilization of elongation factor Tu gene (tuf) sequencing and species-specific PCR (SS-PCR) for the molecular identification of Acetobacter species complex

被引：3

作者：

Huang, Chien-Hsun ^{[1
]}

Chang, Mu-Tzu ^{[2
]}

Huang, Lina ^{[1
]}

Chu, Wen-Shen ^{[1
]}

机构：

[1] Bioresource Collect & Res Ctr, Food Ind Res & Dev Inst, Hsinchu 30062, Taiwan

[2] Natl Hlth Res Inst, Inst Biotechnol & Pharmaceut Res, Zhunan 35053, Miaoli County, Taiwan

来源：

MOLECULAR AND CELLULAR PROBES | 2014年 / 28卷 / 01期

关键词：

Acetobacter complex; tuf sequencing; Comparative sequence analysis species-specific PCR; ACETIC-ACID BACTERIA; REAL-TIME PCR; GLUCONACETOBACTER; DIFFERENTIATION; LACTOBACILLUS; PHYLOGENY; NOV;

D O I：

10.1016/j.mcp.2013.07.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to use tuf gene as a molecular target for species discrimination in the Acetobacter genus, as well as to develop species-specific PCR method for direct species identification of Acetobacter aceti. The results showed that most Acetobacter species could be clearly distinguished, and the average sequence similarity for the tuf gene (89.5%) among type strains was significantly lower than that of the 16S rRNA gene sequence (98.0%). A pair of species-specific primers were designed and used to specifically identify A. aceti, but none of the other Acetobacter strains. Our data indicate that the phylogenetic relationships of most strains in the Acetobacter genus can be resolved using tuf gene sequencing, and the novel species-specific primer pair could be used to rapidly and accurately identify the species of A. aceti by the PCR based assay. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：31 / 33

页数：3

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