Radiosensitizing Effect of Rosmarinic Acid in Metastatic Melanoma B16F10 Cells

被引:1
作者
Alcaraz, Miguel [1 ]
Alcaraz-Saura, Miguel [1 ]
Achel, Daniel Gyingiri [2 ]
Olivares, Amparo [1 ]
Antonio Lopez-Morata, Jose [1 ]
Castillo, Julian [1 ]
机构
[1] Univ Murcia, Radiol & Phys Med Dept, Fac Med Dent, Espinardo 30100, Murcia, Spain
[2] Ghana Atom Energy Commiss, Appl Radiat Biol Ctr, Radiol & Med Sci Res Inst, Legon, Ghana
关键词
Radiation effects; rosmarinic acid; micronuclei; radioprotectors; radiosensitizers; melanoma; PNT2; B16F10; cells; CARNOSIC ACID; ANTIOXIDANT ACTIVITY; CHROMOSOMAL DAMAGE; PHENOLIC-COMPOUNDS; ROSEMARY; MELANOGENESIS; LINES; CONSTITUENTS; OFFICINALIS; DITERPENES;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Rosmarinic acid is an ester of caffeic acid with interesting biological activities including antioxidant effects and scavenging of oxygen-free radicals. Aim: To determine the potentially paradoxical effect of rosmarinic acid, typically being radioprotective when applied to non-tumorous cells, yet conversely displaying a sensitizing action when applied to metastatic B16F10 melanoma cells. Materials and Methods: The genoprotective effect was studied by means of micronucleus tests for anti-mutagenic activity in which the reduction in the frequency of micronuclei was evaluated using cytokinesis-blocked human lymphocytes. The radioprotective effect was studied via a cell viability test in PNT2 (human prostate epithelium) and B16F10 melanoma cells. Results: Rosmarinic acid exhibits a significant genoprotective capacity (p<0.001) against X-rays with a protection factor of 58%, and a dose reduction factor of 7.2. Cell survival obtained after exposure to 10 Gy of X-rays showed a protection factor of 47.5%, thus eliminating 29.1% of radiation-induced cell death in normal prostate epithelial cells (p<0.001). However, in metastatic B16F10 melanoma cells, rosmarinic acid acted not as a radioprotector, but as a sensitizing agent, increasing cellular death by 42% (p<0.001), with an enhancement ratio of 2.36. Conclusion: Rosmarinic acid has an increased capacity for producing radio-induced damage, and thus a paradoxical damaging effect in melanoma cells. Potentially, research into substances such as rosmarinic acid could help clarify mechanisms that provide protection on healthy normal cells, while exclusively damaging neoplastic cells, thus presenting a new strategy for patients undergoing radiotherapy for cancer.
引用
收藏
页码:1913 / 1921
页数:9
相关论文
共 35 条
  • [11] ANTIOXIDANT ACTIVITY OF SOME SPICE ESSENTIAL OILS ON LINOLEIC-ACID OXIDATION IN AQUEOUS-MEDIA
    FARAG, RS
    BADEI, AZMA
    HEWEDI, FM
    ELBAROTY, GSA
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1989, 66 (06) : 792 - 799
  • [12] MEASUREMENT OF MICRONUCLEI IN LYMPHOCYTES
    FENECH, M
    MORLEY, AA
    [J]. MUTATION RESEARCH, 1985, 147 (1-2): : 29 - 36
  • [13] Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion
    Frankel, EN
    Huang, SW
    Aeschbach, R
    Prior, E
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (01) : 131 - 135
  • [14] Hall EJ, RADIODIOLOGY RADIOLO
  • [15] Carnosol inhibits the invasion of B16/F10 mouse melanoma cells by suppressing metalloproteinase-9 through down-regulating nuclear factor-kappaB and c-Jun
    Huang, SC
    Ho, CT
    Lin-Shiau, SY
    Lin, JK
    [J]. BIOCHEMICAL PHARMACOLOGY, 2005, 69 (02) : 221 - 232
  • [16] IAEA, 2011, CYT DOS APPL PREP RE
  • [17] Carnosol: A promising anti-cancer and anti-inflammatory agent
    Johnson, Jeremy J.
    [J]. CANCER LETTERS, 2011, 305 (01) : 1 - 7
  • [18] Castillo JJ, 2010, OLIVES AND OLIVE OIL IN HEALTH AND DISEASE PREVENTION, P951, DOI 10.1016/B978-0-12-374420-3.00102-9
  • [19] Effects of Ephedrae Herba on melanogenesis and gene expression profiles using cDNA microarray in b16 melanocytes
    Kim, Hyeong Jun
    Cho, Yeong Do
    Leem, Kang-Hyun
    Lee, Dong-Nyung
    Kim, Ee-Hwa
    Kim, Myung-Gyou
    Kim, Dae-Keun
    Shin, Tae-Yong
    Boo, Yungmin
    Lee, Je-Hyun
    Kim, Hye Kyung
    [J]. PHYTOTHERAPY RESEARCH, 2006, 20 (09) : 748 - 754
  • [20] Efficacy of Caffeic Acid Phenethyl Ester (CAPE) in skin B16-F0 melanoma tumor bearing C57BL/6 mice
    Kudugunti, Shashi K.
    Vad, Nikhil M.
    Ekogbo, Ehi
    Moridani, Majid Y.
    [J]. INVESTIGATIONAL NEW DRUGS, 2011, 29 (01) : 52 - 62