A Myopathy-linked Desmin Mutation Perturbs Striated Muscle Actin Filament Architecture

被引:42
作者
Conover, Gloria M. [1 ]
Henderson, Syerra N. [1 ]
Gregorio, Carol C. [1 ]
机构
[1] Univ Arizona, Dept Cell Biol & Anat, Tucson, AZ 85724 USA
关键词
SKELETAL-MUSCLE; INTERMEDIATE-FILAMENT; NEBULIN INTERACTS; THIN-FILAMENTS; CARDIAC-MUSCLE; LACKING DESMIN; Z-DISC; GENE; ORGANIZATION; PROTEINS;
D O I
10.1091/mbc.E08-07-0753
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Desmin interacts with nebulin establishing a direct link between the intermediate filament network and sarcomeres at the Z-discs. Here, we examined a desmin mutation, E245D, that is located within the coil IB (nebulin-binding) region of desmin and that has been reported to cause human cardiomyopathy and skeletal muscle atrophy. We show that the coil IB region of desmin binds to C-terminal nebulin (modules 160-164) with high affinity, whereas binding of this desmin region containing the E245D mutation appears to enhance its interaction with nebulin in solid-phase binding assays. Expression of the desmin-E245D mutant in myocytes displaces endogenous desmin and C-terminal nebulin from the Z-discs with a concomitant increase in the formation of intracellular aggregates, reminiscent of a major histological hallmark of desmin-related myopathies. Actin filament architecture was strikingly perturbed in myocytes expressing the desmin-E245D mutant because most sarcomeres contained elongated or shorter actin filaments. Our findings reveal a novel role for desmin intermediate filaments in modulating actin filament lengths and organization. Collectively, these data suggest that the desmin E245D mutation interferes with the ability of nebulin to precisely regulate thin filament lengths, providing new insights into the potential molecular consequences of expression of certain disease-associated desmin mutations.
引用
收藏
页码:834 / 845
页数:12
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