Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes

被引:38
作者
Romano, RA [1 ]
Li, HX [1 ]
Tummala, R [1 ]
Maul, R [1 ]
Sinha, S [1 ]
机构
[1] SUNY Buffalo, Dept Biochem, Buffalo, NY 14214 USA
关键词
basonuclin; zinc finger; cloning; DNA binding; nuclear;
D O I
10.1016/j.ygeno.2003.11.009
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We used a bioinformatics approach to identify Basonuclin2, the second member of the Basonuclin zinc-finger family of transcription factors. The mouse Basonuclin2 protein consists of 1049 amino acids and contains three pairs of zinc fingers in the C-terminus that show a high level of amino acid sequence similarity with Basonuclin 1. In addition, other characteristic domains of Basonuclin 1, such as the serine strip and a nuclear localization signal, are also present in Basonuclin2. We used genomic and in silico database analysis to identify the human and rat homologs of basonuclin2. A search of the mouse genome showed that the hasointclin2 gene maps to chromosome 4 and consists of six exons spanning similar to 300 kb. Northern blot analysis revealed multiple transcripts of basonuclin2 in tissues of the reproductive system (ovary and testis) and also in kidney and skin. We demonstrate that, as expected from sequence conservation, recombinant Basonuclin2 can bind to a sequence in the promoter of a rRNA gene previously characterized as a Basonuclin-binding site. Full-length Basonuclin2 exclusively localizes to the nucleus, indicating that it likely plays an important role in nuclear function, probably in gene regulation. Our study establishes Basonuclin2 as a novel member of the Basonuclin family. Moreover, the structural and functional similarities with Basonuclin I suggest that Basonuclin2 may play an analogous function in germ cells and skin keratinocytes. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:821 / 833
页数:13
相关论文
共 27 条
  • [1] Molecular cloning, chromosomal localization, and expression of the murine SALL1 ortholog Sall1
    Buck, A
    Archangelo, L
    Dixkens, C
    Kohlhase, J
    [J]. CYTOGENETICS AND CELL GENETICS, 2000, 89 (3-4): : 150 - 153
  • [2] Genomic expansion and clustering of ZAD-containing C2H2 zinc-finger genes in Drosophila
    Chung, HR
    Schäfer, U
    Jäckle, H
    Böhm, S
    [J]. EMBO REPORTS, 2002, 3 (12) : 1158 - 1162
  • [3] Glossop NRJ, 1998, J NEUROBIOL, V36, P337, DOI 10.1002/(SICI)1097-4695(19980905)36:3<337::AID-NEU3>3.0.CO
  • [4] 2-X
  • [5] Grummt I, 1999, PROG NUCLEIC ACID RE, V62, P109
  • [6] Alternative subcellular locations of keratinocyte basonuclin
    Iuchi, S
    Easley, K
    Matsuzaki, K
    Weiner, L
    O'Connor, N
    Green, H
    [J]. EXPERIMENTAL DERMATOLOGY, 2000, 9 (03) : 178 - 184
  • [7] Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation
    Iuchi, S
    Green, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (15) : 7948 - 7953
  • [8] Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter
    Iuchi, S
    Green, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (17) : 9628 - 9632
  • [9] NUCLEOLAR TRANSCRIPTION FACTOR HUBF CONTAINS A DNA-BINDING MOTIF WITH HOMOLOGY TO HMG PROTEINS
    JANTZEN, HM
    ADMON, A
    BELL, SP
    TJIAN, R
    [J]. NATURE, 1990, 344 (6269) : 830 - 836
  • [10] SPALT ENCODES AN EVOLUTIONARILY CONSERVED ZINC-FINGER PROTEIN OF NOVEL STRUCTURE WHICH PROVIDES HOMEOTIC GENE-FUNCTION IN THE HEAD AND TAIL REGION OF THE DROSOPHILA EMBRYO
    KUHNLEIN, RP
    FROMMER, G
    FRIEDRICH, M
    GONZALEZGAITAN, M
    WEBER, A
    WAGNERBERNHOLZ, JF
    GEHRING, WJ
    JACKLE, H
    SCHUH, R
    [J]. EMBO JOURNAL, 1994, 13 (01) : 168 - 179