Dynamics of oral microbial community profiling during severe early childhood caries development monitored by PCR-DGGE

被引：28
作者：
Tao, Ye [1 ,2 ]
Zhou, Yan [1 ,2 ]
Ouyang, Yong [1 ,2 ]
Lin, HuanCai [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Dept Prevent Dent, Guangzhou 510275, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Stomatol, Guangzhou, Guangdong, Peoples R China
来源：
ARCHIVES OF ORAL BIOLOGY | 2013年 / 58卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Dental caries; Diversity; Longitudinal study; GRADIENT GEL-ELECTROPHORESIS; SALIVA; RISK; COLONIZATION; STREPTOCOCCI; CHILDREN; DIETARY;
D O I：
10.1016/j.archoralbio.2013.04.005
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: To monitor the longitudinal changes in oral microbial diversity of children with severe early childhood caries (S-EGG) compared to caries free (CF) controls. Methods: Dental plaque samples of 12 children in each group at 8, 14, 20, 26 and 32 months of age were analysed. Total microbial genomic DNA was isolated from each sample, and PCR-denaturing gradient gel electrophoresis (DGGE) analyses were carried out. Results: The number of bands was significantly higher in the CF group (18.17 +/- 4.91 bands) than in the S-ECC group (14.54 +/- 5.56 bands) at 32 months of age (P < 0.05). A trial of 21 genera were identified in all subjects, and there were no significant differences between the two groups at genus level. DGGE profiles showed that most of the clusters were constructed from one individual over time in he both groups. Conclusions: The onset of S-ECC is accompanied by a decrease in microbial diversity. The overall composition of the microbiota is highly similar within an individual over time. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1129 / 1138
页数：10
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