Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation

被引：18
作者：
Chaivisuthangkura, P
Phattanapaijitkul, P
Thammapalerd, N
Rukpratanporn, S
Longyant, S
Sithigorngul, W
Sithigorngul, P [1 ]
机构：
[1] Srinakharinwirot Univ, Fac Sci, Dept Biol, Bangkok 10110, Thailand
[2] Mahidol Univ, Fac Trop Med, Dept Microbiol & Immunol, Bangkok 10400, Thailand
[3] Chulalongkorn Univ, Natl Ctr Genet Engn & Biotechnol, BIOTEC, Ctr Excellence Marine Biotechnol, Bangkok 10330, Thailand
来源：
JOURNAL OF VIROLOGICAL METHODS | 2006年 / 133卷 / 02期
关键词：
immunohistochemistry; penaeus monodon; polyclonal antibody; VP19; western blot; WSSV;
D O I：
10.1016/j.jviromet.2005.11.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：180 / 184
页数：5
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