A novel protein elicitor PeFOC1 from Fusarium oxysporum triggers defense response and systemic resistance in tobacco

被引：18

作者：

Li, Songwei ^{[1
,2
]}

Nie, Haizhen ^{[3
]}

Qiu, Dewen ^{[3
]}

Shi, Mingwang ^{[2
]}

Yuan, Qianhua ^{[1
]}

机构：

[1] Hainan Univ, Inst Trop Agr & Forestry, Hainan Key Lab Sustainable Utilizat Trop Bioresou, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China

[2] Henan Inst Sci & Technol, Sch Resources & Environm Sci, East Sect Hualan Ave, Xinxiang 453003, Henan, Peoples R China

[3] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, 12 Zhong Guan Cun South St, Beijing 100082, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2019年 / 514卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Fusarium oxysporum; Elicitor; PeFOC1; Defense responses; Systemic acquired resistance; SALICYLIC-ACID; PLANT;

D O I：

10.1016/j.bbrc.2019.05.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, it is a hotspot research field on interaction mechanism between elicitor and plant. In this study, a novel hypersensitive response (HR)-inducing protein elicitor was isolated from the culture filtrate of Fusarium oxysporum f. sp. cubense and named PeFOC1, which consisted of 321 amino acids with a molecular weight of approximately 35 kDa. After the inducible expression in Escherichia coli and purification by AKTA explore system, the recombinant PeFOC1 also triggered a typical HR in tobacco. In addition, PeFOC1 induced a cascade of defense response in tobacco including production of hydrogen peroxide, deposition of callose, and accumulation of phenolic compounds. Moreover, PeFOC1 significantly improved systemic resistance of tobacco seedlings to tobacco mosaic virus and Pseudomonas syringae pv. tabaci. Real-time quantitative-PCR analysis indicated that several defense-related genes in tobacco, such as NtPRIa, NtNPR1, NtPAL, NtEDSI, NtPDF, and NtLOX, were all up-regulated by the treatment of PeFOC1. All these results collectively demonstrated that PeFOC1 triggered defense response and systemic acquired resistance (SAR) in tobacco. This research not only provides further research on immune mechanism between plant and elicitor, but also sheds new light on strategy for biocontrol in the future. (C) 2019 Elsevier Inc. All rights reserved.

引用

页码：1074 / 1080

页数：7

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