Bioanalysis of Eukaryotic Organelles

被引:115
作者
Satori, Chad P. [1 ]
Henderson, Michelle M. [1 ]
Krautkramer, Elyse A. [1 ]
Kostal, Vratislav [2 ,3 ]
Distefano, Mark M. [1 ]
Arriaga, Edgar A. [1 ]
机构
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Tescan, Brno 62300, Czech Republic
[3] Inst Analyt Chem ASCR, Brno 60200, Czech Republic
基金
美国国家卫生研究院;
关键词
GREEN FLUORESCENT PROTEIN; ATOMIC-FORCE MICROSCOPY; CAPILLARY ELECTROPHORETIC ANALYSIS; GOLGI NETWORK/EARLY ENDOSOME; ENDOPLASMIC-RETICULUM STRESS; INTEGRAL MEMBRANE-PROTEINS; MASS-SPECTRAL DATA; SUBCELLULAR-LOCALIZATION; OXIDATIVE STRESS; PHAGOSOME MATURATION;
D O I
10.1021/cr300354g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The role that organelle analysis has played in understanding biology is studied. Organelle analysis enables a more specific description of the molecular, biochemical, and physiological processes associated with diseases, embryonic development, tissue differentiation, organism aging, disease treatments, and organism response to pathogens. Confocal microscopy has become a routine tool for investigating subcellular organization, organelle networks, and organelle dynamics in cellular and tissue samples. Most organelles have a dynamic, three-dimensional (3D) organization inside the cell, which is tightly connected to their physiological functions. Due to this, a single 2D image inherently limits the information acquired about the distribution of a particular property within the organelle. The combination of subcellular fractionation with 'omic' technologies has become a powerful resource to characterize and catalogue the various subcellular environments in a cell.
引用
收藏
页码:2733 / 2811
页数:79
相关论文
共 638 条
[1]   Lipid bodies are reservoirs of cyclooxygenase-2 and sites of prostaglandin-E2 synthesis in colon cancer cells [J].
Accioly, Maria T. ;
Pacheco, Patricia ;
Maya-Monteiro, Clarissa M. ;
Carrossini, Nina ;
Robbs, Bruno K. ;
Oliveira, Silvia S. ;
Kaufmann, Cristiane ;
Morgado-Diaz, Jose A. ;
Bozza, Patricia T. ;
Viola, Joao P. B. .
CANCER RESEARCH, 2008, 68 (06) :1732-1740
[2]   Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis [J].
Adam, Rosalyn M. ;
Yang, Wei ;
Di Vizio, Dolores ;
Mukhopadhyay, Nishit K. ;
Steen, Hanno .
BMC CELL BIOLOGY, 2008, 9 (1)
[3]   PLANT ORGANELLE PROTEOMICS: COLLABORATING FOR OPTIMAL CELL FUNCTION [J].
Agrawal, Ganesh Kumar ;
Bourguignon, Jacques ;
Rolland, Norbert ;
Ephritikhine, Genevieve ;
Ferro, Myriam ;
Jaquinod, Michel ;
Alexiou, Konstantinos G. ;
Chardot, Thierry ;
Chakraborty, Niranjan ;
Jolivet, Pascale ;
Doonan, John H. ;
Rakwal, Randeep .
MASS SPECTROMETRY REVIEWS, 2011, 30 (05) :772-853
[4]   On-column labeling for capillary electrophoretic analysis of individual mitochondria directly sampled from tissue cross sections [J].
Ahmadzadeh, H ;
Thompson, LV ;
Arriaga, EA .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (01) :169-174
[5]   Synaptotagmin IV is necessary for the maturation of secretory granules in PC12 cells [J].
Ahras, M ;
Otto, GP ;
Tooze, SA .
JOURNAL OF CELL BIOLOGY, 2006, 173 (02) :241-251
[6]   In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis [J].
Albrecht, Simone C. ;
Barata, Ana Gomes ;
Grosshans, Joerg ;
Teleman, Aurelio A. ;
Dick, Tobias P. .
CELL METABOLISM, 2011, 14 (06) :819-829
[7]   3-NITROPROPIONATE, TOXIC SUBSTANCE OF INDIGOFERA, IS A SUICIDE INACTIVATOR OF SUCCINATE-DEHYDROGENASE - (RAT-LIVER MITOCHONDRIA CARBANION-N-5 FLAVIN ADDUCTS 2-PROTON ABSTRACTION MECHANISM) [J].
ALSTON, TA ;
MELA, L ;
BRIGHT, HJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (09) :3767-3771
[8]   Trends in ultrasensitive proteomics [J].
Altelaar, A. F. Maarten ;
Heck, Albert J. R. .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2012, 16 (1-2) :206-213
[9]   Proteomic characterization of the human centrosome by protein correlation profiling [J].
Andersen, JS ;
Wilkinson, CJ ;
Mayor, T ;
Mortensen, P ;
Nigg, EA ;
Mann, M .
NATURE, 2003, 426 (6966) :570-574
[10]   Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum [J].
Anderson, Daniel J. ;
Hetzer, Martin W. .
NATURE CELL BIOLOGY, 2007, 9 (10) :1160-1166