Ebolavirus VP35 Coats the Backbone of Double-Stranded RNA for Interferon Antagonism

被引：37

作者：

Bale, Shridhar ^{[1
]}

Julien, Jean-Philippe ^{[2
]}

Bornholdt, Zachary A. ^{[1
]}

Krois, Alexander S. ^{[2
]}

Wilson, Ian A. ^{[2
,3
]}

Saphire, Erica Ollmann ^{[1
,3
]}

机构：

[1] Scripps Res Inst, Dept Immunol & Microbial Sci, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 18期

基金：

加拿大健康研究院;

关键词：

STRUCTURAL BASIS; DSRNA RECOGNITION; PROTEIN; INFECTION; RESPONSES; MODEL;

D O I：

10.1128/JVI.01452-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recognition of viral double-stranded RNA (dsRNA) activates interferon production and immune signaling in host cells. Crystal structures of ebolavirus VP35 show that it caps dsRNA ends to prevent sensing by pattern recognition receptors such as RIG-I. In contrast, structures of marburgvirus VP35 show that it primarily coats the dsRNA backbone. Here, we demonstrate that ebolavirus VP35 also coats the dsRNA backbone in solution, although binding to the dsRNA ends probably constitutes the initial binding event.

引用

页码：10385 / 10388

页数：4

共 29 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].

Adams, Paul D. ;

Afonine, Pavel V. ;

Bunkoczi, Gabor ;

Chen, Vincent B. ;

Davis, Ian W. ;

Echols, Nathaniel ;

Headd, Jeffrey J. ;

Hung, Li-Wei ;

Kapral, Gary J. ;

Grosse-Kunstleve, Ralf W. ;

McCoy, Airlie J. ;

Moriarty, Nigel W. ;

Oeffner, Robert ;

Read, Randy J. ;

Richardson, David C. ;

Richardson, Jane S. ;

Terwilliger, Thomas C. ;

Zwart, Peter H. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :213-221

[2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[3] Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients [J].

Baize, S ;

Leroy, EM ;

Georges-Courbot, MC ;

Capron, M ;

Lansoud-Soukate, J ;

Debré, P ;

Fisher-Hoch, SP ;

McCormick, JB ;

Georges, AJ .

NATURE MEDICINE, 1999, 5 (04) :423-426

[4] Marburg Virus VP35 Can Both Fully Coat the Backbone and Cap the Ends of dsRNA for Interferon Antagonism [J].

Bale, Shridhar ;

Julien, Jean-Philippe ;

Bornholdt, Zachary A. ;

Kimberlin, Christopher R. ;

Halfmann, Peter ;

Zandonatti, Michelle A. ;

Kunert, John ;

Kroon, Gerard J. A. ;

Kawaoka, Yoshihiro ;

MacRae, Ian J. ;

Wilson, Ian A. ;

Saphire, Erica Ollmann .

PLOS PATHOGENS, 2012, 8 (09)

[5] Structural Basis for Differential Neutralization of Ebolaviruses [J].

Bale, Shridhar ;

Dias, Joao M. ;

Fusco, Marnie L. ;

Hashiguchi, Takao ;

Wong, Anthony C. ;

Liu, Tong ;

Keuhne, Ana I. ;

Li, Sheng ;

Woods, Virgil L., Jr. ;

Chandran, Kartik ;

Dye, John M. ;

Saphire, Erica Ollmann .

VIRUSES-BASEL, 2012, 4 (04) :447-470

[6] The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3 [J].

Basler, CF ;

Mikulasova, A ;

Martinez-Sobrido, L ;

Paragas, J ;

Mühlberger, E ;

Bray, M ;

Klenk, HD ;

Palese, P ;

García-Sastre, A .

JOURNAL OF VIROLOGY, 2003, 77 (14) :7945-7956

[7] Structural basis of innate immune recognition of viral RNA [J].

Berke, Ian C. ;

Li, Yue ;

Modis, Yorgo .

CELLULAR MICROBIOLOGY, 2013, 15 (03) :386-394

[8] Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I) [J].

Binder, Marco ;

Eberle, Florian ;

Seitz, Stefan ;

Muecke, Norbert ;

Hueber, Christian M. ;

Kiani, Narsis ;

Kaderali, Lars ;

Lohmann, Volker ;

Dalpke, Alexander ;

Bartenschlager, Ralf .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (31) :27278-27287

[9] Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling [J].

Cardenas, Washington B. ;

Loo, Yueh-Ming ;

Gale, Michael, Jr. ;

Hartman, Amy L. ;

Kimberlin, Christopher R. ;

Martinez-Sobrido, Luis ;

Saphire, Erica Ollmann ;

Basler, Christopher F. .

JOURNAL OF VIROLOGY, 2006, 80 (11) :5168-5178

[10] Dual modes of RNA-silencing suppression by flock house virus protein B2 [J].

Chao, JA ;

Lee, JH ;

Chapados, BR ;

Debler, EW ;

Schneemann, A ;

Williamson, JR .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (11) :952-957

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