RETENTION MECHANISM IN ZWITTERIONIC HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (ZIC-HILIC) STUDIED FOR HIGHLY POLAR COMPOUNDS UNDER DIFFERENT ELUTION CONDITIONS

被引：0

作者：

Bacalum, Elena ^{[1
]}

Tanase, Maria ^{[2
]}

Cheregi, Mihaela ^{[2
]}

Aboul-Enein, Hassan Y. ^{[3
]}

David, Victor ^{[2
]}

机构：

[1] Univ Bucharest, Res Inst, ICUB, Blvd M Kogalniceanu,36-46, Bucharest 050107, Romania

[2] Univ Bucharest, Fac Chem, Dept Analyt Chem, Sos Panduri,90, Bucharest 050663, Romania

[3] Natl Res Ctr, Pharmaceut & Med Chem Dept, Pharmaceut & Drug Ind Res Div, Cairo 12311, Egypt

来源：

REVUE ROUMAINE DE CHIMIE | 2016年 / 61卷 / 6-7期

关键词：

ZIC-HILIC; sulfobetaine; polar compounds; retention mechanism; HIGH-PURITY SILICA; STATIONARY PHASES; SEPARATION; BEHAVIOR; SELECTIVITY; COLUMN; ACIDS; EPIRUBICIN; PEPTIDES; SOLUTES;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The retention behavior of six highly polar compounds on zwitterionic sulfobetaine stationary phase was studied, under different elution conditions. The influence of organic modifier type (acetonitrile, methanol), the organic solvent content, ammonium acetate concentration added to the mobile phase and column temperature on the retention of studied compounds were investigated. Influence of temperature on the retention of studied compounds was also investigated and van't Hoff pious were used in estimating thermodynamic parameters of the chromatographic process. Two mathematical models usually used to differentiate between partition and adsorption processes were applied to the dependences of retention factor on mobile phase composition. These obtained results suggest that a mixed retention mechanism relying on both partition and adsorption processes may contribute to the chromatographic behavior of studied compounds on ZIC-HILIC stationary phase.

引用

页码：531 / 539

页数：9

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