Clinical use of quantitative molecular methods in studying human immunodeficiency virus type 1 infection

被引：28

作者：

Clementi, M ^{[1
]}

Menzo, S ^{[1
]}

Bagnarelli, P ^{[1
]}

Valenza, A ^{[1
]}

Paolucci, S ^{[1
]}

Sampaolesi, R ^{[1
]}

Manzin, A ^{[1
]}

Varaldo, PE ^{[1
]}

机构：

[1] UNIV TRIESTE, DIPARTIMENTO SCI BIOMED, TRIESTE, ITALY

来源：

CLINICAL MICROBIOLOGY REVIEWS | 1996年 / 9卷 / 02期

关键词：

D O I：

10.1128/CMR.9.2.135

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The absolute quantitation of specific human immunodeficiency virus type 1 (HIV-1) RNA and DNA sequences in clinical specimens from infected persons with different clinical conditions is necessary, at present, to study the natural history and pathogenicity of HIV-1 infection and to allow rational medical management of treated and untreated HIV-1 patients. Recently, methods for semiquantitative and quantitative analysis of HIV-1 nucleic acid sequences have been developed and cross-sectional and longitudinal studies have been performed to study the HIV-1 load and activity in samples from infected patients by quantitative molecular assays. The data obtained with these researches have shown that a direct correlation exists between viral load and disease progression. More recently, pilot studies demonstrated the ability of quantitative molecular methods to monitor specific anti-HIV-1 therapies in real time. All this suggests that the opportunity of quantifying specific viral RNA and DNA species increases significantly the diagnostic potential of virological laboratories in studying HIV-1-infected persons.

引用

页码：135 / +

页数：1

共 176 条

[101] COMPLEX INTRAPATIENT SEQUENCE VARIATION IN THE V1 AND V2 HYPERVARIABLE REGIONS OF THE HIV-1 GP120 ENVELOPE SEQUENCE [J].