Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

被引：16
|
作者：
Kong, Kenny [1 ]
Rowlands, Christopher J. [1 ]
Elsheikha, Hany [2 ]
Notingher, Ioan [1 ]
机构：
[1] Univ Nottingham, Sch Phys & Astron, Nottingham NG7 2RD, England
[2] Univ Nottingham, Sch Vet Med & Sci, Nottingham LE12 5RD, England
来源：
ANALYST | 2012年 / 137卷 / 18期
基金：
美国国家卫生研究院;
关键词：
EMBRYONIC STEM-CELLS; MICROSPECTROSCOPY; DYNAMICS; MASS; RNA;
D O I：
10.1039/c2an35640f
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at similar to 10x shorter acquisition time compared to raster scanning.
引用
收藏
页码：4119 / 4122
页数：4
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